Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4631759..4632256 Replicon chromosome
Accession NZ_CP091664
Organism Escherichia coli strain KKa019

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag L6L56_RS22510 Protein ID WP_001173343.1
Coordinates 4631759..4631932 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag L6L56_RS22515 Protein ID WP_000398619.1
Coordinates 4631960..4632256 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6L56_RS22500 (4628429) 4628429..4629886 + 1458 WP_001550473.1 dipeptide/tripeptide permease DtpC -
L6L56_RS22505 (4630123) 4630123..4631640 + 1518 WP_001550472.1 lysine--tRNA ligase -
L6L56_RS22510 (4631759) 4631759..4631932 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
L6L56_RS22515 (4631960) 4631960..4632256 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
L6L56_RS22520 (4632485) 4632485..4632757 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
L6L56_RS22525 (4632769) 4632769..4632999 - 231 WP_001550470.1 4Fe-4S mono-cluster protein YjdI -
L6L56_RS22530 (4633179) 4633179..4634810 + 1632 WP_001216477.1 sensor histidine kinase -
L6L56_RS22535 (4634807) 4634807..4635526 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T234379 WP_001173343.1 NZ_CP091664:c4631932-4631759 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T234379 NZ_LR130533:5786365-5786823 [Pseudomonas aeruginosa]
ATGCAACGGCATGGCTGGACGCTGCTCTTCCACGACTGCGTGATCGAGCAGTTGCAGAAACTGCATGCGGCCGCGAGGCG
CGCGCAGGAGAATGACCCGGCGGGCTTCGAGTCCAATGCCAACGTCAAGCTCTTCCGGGCCCTGAGCCAGTTGATGTTGG
ATGTGGTGCCAGGCGATCCGGCACGCGACGAGTACCGCCAGGGCAACACCTTGGGACCTGCCCACCGCCACTGGCGAAGG
GCCAAGATCGGAAGGCGGTTCCGGCTGTTCTTCCGGTACGACTCGAAGGCGAAGGTCATTGTGTACGCCTGGGTCAACGA
TGAACAGACCCTGCGGTCTTCGGGTAGCAAATCGGACCCGTATGTCGTGTTCGAGAAGATGCTCGGACGCGGGAACCCAC
CAGACGACTGGCACGCGCTGATACAGGCAAGCAAGCAGGATTGGAGCAAACTGGAATAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT234379 WP_000398619.1 NZ_CP091664:c4632256-4631960 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT234379 NZ_LR130533:5786048-5786365 [Pseudomonas aeruginosa]
ATGCCTGCCATCCACGAAGTCGCCACGTTGACCTCCAAGGGCCAGGTCACGCTGCCCAAGCCTATCCGGCAGGCGCTGGG
CGTTGATGCCGGCGGCAAGCTCGCGTTCGATCTGCGGGGCAGCGAAGTCGTCGTCACCCGTGTTGATGCCGAGCACGAGG
ACCCCGCCATTGGCGCGTTTCTGAGCCTGCTGGCCCGCGACATCGAAGCTGGCCGGAACGTCCGGGGCCTGCCCGAGGAT
CTGGCGCGCGCCATGCTGGAGCATGCTGGCCACGGTGTGAACCTGGACGAGGAGATCGACGGGAAAGTGGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References