Detailed information of TA system TA234372

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 3103384..3104168
• Replicon: chromosome
• Accession: NZ_CP091664
• Organism: Escherichia coli strain KKa019

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: V0T0H9
• Locus tag: L6L56_RS15245
• Protein ID: WP_000613626.1
• Coordinates: 3103674..3104168 (+)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: L4JCW6
• Locus tag: L6L56_RS15240
• Protein ID: WP_001110447.1
• Coordinates: 3103384..3103677 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L6L56_RS15230 (3098534)	3098534..3099493	-	960	WP_000846342.1	23S rRNA pseudouridine(955/2504/2580) synthase RluC	-
L6L56_RS15235 (3100066)	3100066..3103251	+	3186	WP_001550951.1	ribonuclease E	-
L6L56_RS15240 (3103384)	3103384..3103677	+	294	WP_001110447.1	DUF1778 domain-containing protein	Antitoxin
L6L56_RS15245 (3103674)	3103674..3104168	+	495	WP_000613626.1	GNAT family N-acetyltransferase	Toxin
L6L56_RS15250 (3104263)	3104263..3105216	-	954	WP_001212768.1	flagellar hook-associated protein FlgL	-
L6L56_RS15255 (3105228)	3105228..3106871	-	1644	WP_000096478.1	flagellar hook-associated protein FlgK	-
L6L56_RS15260 (3106937)	3106937..3107878	-	942	WP_001366226.1	flagellar assembly peptidoglycan hydrolase FlgJ	-
L6L56_RS15265 (3107878)	3107878..3108975	-	1098	WP_001441925.1	flagellar basal body P-ring protein FlgI	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (81-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 18084.00 Da
• Isoelectric Point: 8.2617

>T234372 WP_000613626.1 NZ_CP091664:3103674-3104168 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Nucleotide

• Length: 495 bp

>T234372 NZ_LR130533:c37430-37053 [Pseudomonas aeruginosa]
ATGACAAACAAAGAAAAACCGCTCGAATGGATCGCGAGCAGCCACAAGGATTTGATGGCGTTGCCGTCCGACGTGCGTCG
CCGTTTCGGTTACGCGCTCTCGTTGGCGCAGATAGGCGATCAGGATGACGCAGCAAAGGTGCTCAAGGGGTTCGGTGGTG
CCGGCGTGCTGGAGGTCGTCGAAGACGATGCCGGCGGCACCTATCGAGCGGTATACACGGTCAAGTTTGCGGAAGCGGTG
TTCGTCCTGCACTGCTTCCAGAAGAAGAGCAAGAGCGGAATCGCCACGCCAAAGGCCGACATGGACATCATCCGCGCTCG
GCTGAAGGTGGCCGAGGTATTGGCACAGGAGCTACGAAATGCAAAAACGAATCATTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11109.69 Da
• Isoelectric Point: 10.5423

>AT234372 WP_001110447.1 NZ_CP091664:3103384-3103677 [Escherichia coli]
MPAANNIPTRRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT234372 NZ_LR130533:c37072-36737 [Pseudomonas aeruginosa]
ATGCAAAAACGAATCATTGAAGGCGTCGAGGTTCAGCGCAGCTCGGGCAACGTCTTTGCCGACCTTGGACTGCCTGACGC
TGAAAAGCTCAAGATCAAGACCGGCCTGGTGGTCGAGATCAGGAGGGCCATGCGCGCCCTTGGGCTGACTCAACAAGCGG
CGGCCAAACGCATGGGCATCCCGCAACCGAAGGTGTCGGGCATGATGCGCGGCGACTTCACCAATCTATCCGAACGCAAG
CTGATGGATTGTCTGAATCGCCTCGGCTACGACATCGAAATCAAGGTACGGCCAGCAGCCGAGCCGATCGGGCATCTAAC
GCTCGCAACCGCTTAA

Structures

Toxin

• AlphaFold DB: V0T0H9

Antitoxin

• AlphaFold DB: L4JCW6