Detailed information of TA system TA234371

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2667688..2668326
• Replicon: chromosome
• Accession: NZ_CP091664
• Organism: Escherichia coli strain KKa019

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A7U9DHD1
• Locus tag: L6L56_RS12880
• Protein ID: WP_000813795.1
• Coordinates: 2668150..2668326 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: L6L56_RS12875
• Protein ID: WP_076797675.1
• Coordinates: 2667688..2668104 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L6L56_RS12855 (2662840)	2662840..2663781	-	942	WP_106106616.1	ABC transporter permease	-
L6L56_RS12860 (2663782)	2663782..2664795	-	1014	WP_001551095.1	ABC transporter ATP-binding protein	-
L6L56_RS12865 (2664813)	2664813..2665958	-	1146	WP_001551094.1	ABC transporter substrate-binding protein	-
L6L56_RS12870 (2666203)	2666203..2667609	-	1407	WP_001551093.1	PLP-dependent aminotransferase family protein	-
L6L56_RS12875 (2667688)	2667688..2668104	-	417	WP_076797675.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
L6L56_RS12880 (2668150)	2668150..2668326	-	177	WP_000813795.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
L6L56_RS12885 (2668548)	2668548..2668778	+	231	WP_023910283.1	YncJ family protein	-
L6L56_RS12890 (2668870)	2668870..2670831	-	1962	WP_001551090.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
L6L56_RS12895 (2670904)	2670904..2671440	-	537	WP_001551089.1	DNA-binding transcriptional regulator SutR	-
L6L56_RS12900 (2671532)	2671532..2672704	+	1173	WP_001551088.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-65)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6747.80 Da
• Isoelectric Point: 11.5666

>T234371 WP_000813795.1 NZ_CP091664:c2668326-2668150 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPSEEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T234371 NZ_LR130532:c4537431-4537156 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATAGTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGCAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15220.55 Da
• Isoelectric Point: 4.7386

>AT234371 WP_076797675.1 NZ_CP091664:c2668104-2667688 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPSNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT234371 NZ_LR130532:c4537159-4536800 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Structures

Toxin

• AlphaFold DB: A0A7U9DHD1