Detailed information of TA system TA023437

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4412784..4413618
• Replicon: chromosome
• Accession: NC_011750
• Organism: Escherichia coli IAI39

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A8T5WFL4
• Locus tag: ECIAI39_RS21275
• Protein ID: WP_000854823.1
• Coordinates: 4413241..4413618 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A8T5WFE1
• Locus tag: ECIAI39_RS21270
• Protein ID: WP_001285614.1
• Coordinates: 4412784..4413152 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECIAI39_RS21235	4408504..4409184	+	681	WP_001278651.1	WYL domain-containing protein	-
ECIAI39_RS21240	4409335..4410012	+	678	WP_001097562.1	hypothetical protein	-
ECIAI39_RS21245	4410018..4410251	+	234	WP_000883181.1	DUF905 family protein	-
ECIAI39_RS21250	4410341..4411159	+	819	WP_001175143.1	DUF945 domain-containing protein	-
ECIAI39_RS21255	4411425..4411904	+	480	WP_000706978.1	antirestriction protein	-
ECIAI39_RS21260	4411920..4412396	+	477	WP_001186792.1	RadC family protein	-
ECIAI39_RS21265	4412483..4412704	+	222	WP_000692350.1	DUF987 domain-containing protein	-
ECIAI39_RS21270	4412784..4413152	+	369	WP_001285614.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
ECIAI39_RS21275	4413241..4413618	+	378	WP_000854823.1	TA system toxin CbtA family protein	Toxin
ECIAI39_RS21280	4413615..4413764	+	150	Protein_4161	hypothetical protein	-
ECIAI39_RS21285	4413840..4414037	+	198	WP_087890155.1	DUF957 domain-containing protein	-
ECIAI39_RS21290	4414122..4414964	+	843	WP_001290170.1	DUF4942 domain-containing protein	-
ECIAI39_RS21295	4415201..4415329	+	129	Protein_4164	virulence RhuM family protein	-
ECIAI39_RS21300	4415758..4416942	+	1185	WP_001172866.1	sugar efflux transporter	-
ECIAI39_RS21305	4417053..4417976	+	924	WP_000535959.1	carboxylate/amino acid/amine transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14072.04 Da
• Isoelectric Point: 6.7093

>T23437 WP_000854823.1 NC_011750:4413241-4413618 [Escherichia coli IAI39]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCTHSQLINSIDILRARRATGLMTRDNYRMVNDISQGKYPEAKQ

Nucleotide

• Length: 378 bp

>T23437 NC_011750:4413241-4413618 [Escherichia coli IAI39]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCACGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGATCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCACCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCCCGCCGGGCGACAGGCCTGATGACCCGCGA
CAATTACAGAATGGTAAATGACATTAGCCAGGGGAAGTATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13596.35 Da
• Isoelectric Point: 6.0618

>AT23437 WP_001285614.1 NC_011750:4412784-4413152 [Escherichia coli IAI39]
VSDTLPGTTLPDDNNDRTWWGLPCTVTSCFGARLVQEGNRLHYLADRAGIRGRFSDADSYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT23437 NC_011750:4412784-4413152 [Escherichia coli IAI39]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAATAACGACCGCACCTGGTGGGGACTGCCCTGCACCGTGAC
GTCCTGTTTTGGTGCCCGTCTGGTACAGGAAGGCAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGGCGGT
TCAGCGACGCGGATTCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA