Detailed information of TA system TA023436

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 4125835..4126466
• Replicon: chromosome
• Accession: NC_011750
• Organism: Escherichia coli IAI39

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A8T5W4M7
• Locus tag: ECIAI39_RS19970
• Protein ID: WP_001260303.1
• Coordinates: 4126191..4126466 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: U9Y7E1
• Locus tag: ECIAI39_RS19965
• Protein ID: WP_000593555.1
• Coordinates: 4125835..4126194 (-)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECIAI39_RS19935	4120950..4121423	+	474	WP_001161554.1	PTS sugar transporter subunit IIA	-
ECIAI39_RS19940	4121420..4121701	+	282	WP_000084021.1	PTS sugar transporter subunit IIB	-
ECIAI39_RS19945	4121775..4123133	+	1359	WP_001302220.1	PTS galactitol transporter subunit IIC	-
ECIAI39_RS19950	4123126..4124634	+	1509	WP_012602556.1	carbohydrate kinase	-
ECIAI39_RS19955	4124624..4124893	+	270	WP_000949581.1	HPr family phosphocarrier protein	-
ECIAI39_RS19960	4124925..4125785	+	861	WP_001131789.1	class II aldolase	-
ECIAI39_RS19965	4125835..4126194	-	360	WP_000593555.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
ECIAI39_RS19970	4126191..4126466	-	276	WP_001260303.1	type II toxin-antitoxin system HicA family toxin	Toxin
ECIAI39_RS19975	4126539..4127663	-	1125	WP_001314210.1	ABC transporter permease	-
ECIAI39_RS19980	4127663..4130398	-	2736	WP_000149108.1	ribosome-associated ATPase/putative transporter RbbA	-
ECIAI39_RS19985	4130395..4131462	-	1068	WP_000361489.1	HlyD family secretion protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (30-80)

Antitoxin

• Uncharacterised protein family HicB (60-112)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10167.85 Da
• Isoelectric Point: 10.6128

>T23436 WP_001260303.1 NC_011750:c4126466-4126191 [Escherichia coli IAI39]
MRTQVQALRKKQKNTLDQIVKSPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKSAVESV
RDWLLSIGVKP

Nucleotide

• Length: 276 bp

>T23436 NC_011750:c4126466-4126191 [Escherichia coli IAI39]
ATGAGAACCCAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATAGTTAAATCTCCTGTTCCTCAAGG
GATTAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAAGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13402.27 Da
• Isoelectric Point: 5.1987

>AT23436 WP_000593555.1 NC_011750:c4126194-4125835 [Escherichia coli IAI39]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Nucleotide

• Length: 360 bp

>AT23436 NC_011750:c4126194-4125835 [Escherichia coli IAI39]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AGCTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACATTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTAAATACTTA
TATTATTGAAACGCTAAATGAGCGTTTGAATCATTTATAA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LKZ6