Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 3337912..3338495 Replicon chromosome
Accession NC_011750
Organism Escherichia coli IAI39

Toxin (Protein)


Gene name mazF Uniprot ID S1EZP4
Locus tag ECIAI39_RS16135 Protein ID WP_000254738.1
Coordinates 3337912..3338247 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A1M2IZI3
Locus tag ECIAI39_RS16140 Protein ID WP_000581940.1
Coordinates 3338247..3338495 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECIAI39_RS16120 3333799..3335097 - 1299 WP_000036723.1 phosphopyruvate hydratase -
ECIAI39_RS16125 3335184..3336821 - 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
ECIAI39_RS16130 3337049..3337840 - 792 WP_001071660.1 nucleoside triphosphate pyrophosphohydrolase -
ECIAI39_RS16135 3337912..3338247 - 336 WP_000254738.1 endoribonuclease MazF Toxin
ECIAI39_RS16140 3338247..3338495 - 249 WP_000581940.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
ECIAI39_RS16145 3338573..3340807 - 2235 WP_000226815.1 GTP pyrophosphokinase -
ECIAI39_RS16150 3340855..3342156 - 1302 WP_000046807.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
ECIAI39_RS16155 3342213..3343427 + 1215 Protein_3154 HAMP domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 3332345..3333673 1328


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12098.04 Da        Isoelectric Point: 8.2618

>T23432 WP_000254738.1 NC_011750:c3338247-3337912 [Escherichia coli IAI39]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T23432 NC_011750:c3338247-3337912 [Escherichia coli IAI39]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTCAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTTGAAGTTGTTCTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTGCTGATTGGGTAG

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9270.67 Da        Isoelectric Point: 4.5972

>AT23432 WP_000581940.1 NC_011750:c3338495-3338247 [Escherichia coli IAI39]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIGLVDGKLIIEPVRKEPVFTLAELVSDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT23432 NC_011750:c3338495-3338247 [Escherichia coli IAI39]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCTCCTGCAGTGCGGATCCCAGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGGCCTGGTGGATGGCAAATTAATTATTGAGCCAGTACGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAGCGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGGGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CK9
PDB 5CKH
PDB 5CO7
PDB 3NFC
PDB 5CR2
PDB 5CKB
PDB 5CQY
PDB 5CKD
PDB 5CKE
PDB 5CKF

Antitoxin

Source ID Structure
AlphaFold DB A0A1M2IZI3

References