234316

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 3208846..3209683 Replicon chromosome
Accession NZ_CP091659
Organism Escherichia coli strain KFu024

Toxin (Protein)


Gene name itaT Uniprot ID Q3Z4X7
Locus tag L6L61_RS16445 Protein ID WP_000227784.1
Coordinates 3208846..3209388 (-) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag L6L61_RS16450 Protein ID WP_001297137.1
Coordinates 3209372..3209683 (-) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6L61_RS16425 (3204385) 3204385..3205296 - 912 WP_000705874.1 2-dehydropantoate 2-reductase -
L6L61_RS16430 (3205464) 3205464..3205955 + 492 WP_001138904.1 nucleotide binding protein YajQ -
L6L61_RS16435 (3206083) 3206083..3207447 - 1365 WP_001000978.1 MFS transporter -
L6L61_RS16440 (3207855) 3207855..3208790 + 936 WP_001297127.1 tetratricopeptide repeat protein -
L6L61_RS16445 (3208846) 3208846..3209388 - 543 WP_000227784.1 GNAT family N-acetyltransferase Toxin
L6L61_RS16450 (3209372) 3209372..3209683 - 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
L6L61_RS16455 (3209868) 3209868..3210758 - 891 WP_000971336.1 heme o synthase -
L6L61_RS16460 (3210770) 3210770..3211099 - 330 WP_113396804.1 cytochrome o ubiquinol oxidase subunit IV -
L6L61_RS16465 (3211099) 3211099..3211713 - 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
L6L61_RS16470 (3211703) 3211703..3213694 - 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
L6L61_RS16475 (3213716) 3213716..3214663 - 948 WP_001239441.1 cytochrome o ubiquinol oxidase subunit II -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19805.02 Da        Isoelectric Point: 8.3395

>T234316 WP_000227784.1 NZ_CP091659:c3209388-3208846 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T234316 NZ_LR130515:c2422850-2422746 [Staphylococcus aureus]
TTGTTACTCCTAGAAAGGACTAGCATGTCTGATTTTGAAATGCTTATGGTTGTATTAACAATCATTGGTTTAGTATTGAT
TAGTACTCAAGACCATAAAAAATAA

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT234316 WP_001297137.1 NZ_CP091659:c3209683-3209372 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT234316 NZ_LR130515:2422633-2422688 [Staphylococcus aureus]
AAAAAGGGCAACACTCGGAAACATGTTACCCTAATGAGCCCGTTAAAAAGACGGTG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7BYY

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References