Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 2695619..2696195 Replicon chromosome
Accession NZ_CP091659
Organism Escherichia coli strain KFu024

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag L6L61_RS13980 Protein ID WP_001297643.1
Coordinates 2695619..2695906 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A080I7H7
Locus tag L6L61_RS13985 Protein ID WP_000063160.1
Coordinates 2695893..2696195 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6L61_RS13965 (2691856) 2691856..2693136 - 1281 WP_001037390.1 DUF445 domain-containing protein -
L6L61_RS13970 (2693177) 2693177..2694409 - 1233 WP_001137030.1 multidrug efflux MFS transporter MdtM -
L6L61_RS13975 (2694724) 2694724..2695420 + 697 Protein_2556 IS1 family transposase -
L6L61_RS13980 (2695619) 2695619..2695906 + 288 WP_001297643.1 BrnT family toxin Toxin
L6L61_RS13985 (2695893) 2695893..2696195 + 303 WP_000063160.1 BrnA antitoxin family protein Antitoxin
L6L61_RS13990 (2696229) 2696229..2697185 - 957 WP_001297640.1 GTPase -
L6L61_RS13995 (2697196) 2697196..2697399 - 204 WP_000467859.1 YbdD/YjiX family protein -
L6L61_RS14000 (2697449) 2697449..2699599 - 2151 WP_001295524.1 pyruvate/proton symporter BtsT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 2695211..2695420 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T234313 WP_001297643.1 NZ_CP091659:2695619-2695906 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T234313 NZ_LR130515:c2224895-2224500 [Staphylococcus aureus]
ATGCAAAACATTAAATATTTAACTGAAAAACAAGTAATTGCCATTAATGTAAAAGCAATTCAAGAACTCTCACCTAAAGA
ACAAGTTGGTGTTAAAGTTCCAGAGGTTCTTAATGCTACTATTGAAGGAGTTAAACAATCATTTGGTGGAGTTGAACTAT
ATGAAACAATCGAGAGAAAAGCGGCTTTTATATACAGAAATATAGCTCAAAAACATGCATTCTTTAATGCGAATAAGAGA
ACAGCTTTTACATCTATGGTTATTTTCTTGAAGTTAAATAAGATTAATTTTAATTGTACTCAAGACGAAGCGGTACAATT
TACTTTAAAAGTGGTAGAGGATAAAACTCTTACACTTGAAGATATTGCAGATTGGATCAAAAGGCATTGTAAATAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11552.01 Da        Isoelectric Point: 9.4646

>AT234313 WP_000063160.1 NZ_CP091659:2695893-2696195 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALTNKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT234313 NZ_LR130515:c2225125-2224895 [Staphylococcus aureus]
ATGGTTACTACAAGAAAACTAAGAAGAGCTGGCAATAGTTCAGTTGTTTCTGTACCAACAGAAGTGATCGCAGCACTCGG
AGTTACAAATGGTGACAATCTTAAATTTAATATTGAAGATAATAAAGTAACCATTGAAAAGGAGGTAAACGAAGACGAAG
AGTTTTTCAAATTGTTGGATGAAACATTCACTGAGTATAACCAAGCTCTGAGAAAGTTGGTTGACATATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A080I7H7

References