Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 2432126..2432623 Replicon chromosome
Accession NZ_CP091659
Organism Escherichia coli strain KFu024

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag L6L61_RS12615 Protein ID WP_001173343.1
Coordinates 2432450..2432623 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag L6L61_RS12610 Protein ID WP_000398619.1
Coordinates 2432126..2432422 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6L61_RS12590 (2428857) 2428857..2429576 - 720 WP_000611288.1 two-component system response regulator DcuR -
L6L61_RS12595 (2429573) 2429573..2431204 - 1632 WP_001216477.1 sensor histidine kinase -
L6L61_RS12600 (2431385) 2431385..2431615 + 231 WP_024250366.1 4Fe-4S mono-cluster protein YjdI -
L6L61_RS12605 (2431627) 2431627..2431899 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
L6L61_RS12610 (2432126) 2432126..2432422 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
L6L61_RS12615 (2432450) 2432450..2432623 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
L6L61_RS12625 (2434061) 2434061..2435578 - 1518 WP_001295074.1 lysine--tRNA ligase -
L6L61_RS12630 (2435815) 2435815..2437272 - 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T234310 WP_001173343.1 NZ_CP091659:2432450-2432623 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T234310 NZ_LR130515:1957923-1958018 [Staphylococcus aureus]
GTGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAG
ACGCAATACAAAATAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT234310 WP_000398619.1 NZ_CP091659:2432126-2432422 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT234310 NZ_LR130515:c1958105-1958046 [Staphylococcus aureus]
ACAACGAAAATAAGTATTTACTTATACACCAATCCCCTCACTATTTGCGGTAGTGAGGGG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References