Detailed information of TA system TA023431

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 2125648..2126432
• Replicon: chromosome
• Accession: NC_011750
• Organism: Escherichia coli IAI39

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: ECIAI39_RS10480
• Protein ID: WP_000613629.1
• Coordinates: 2125938..2126432 (+)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: B1LI61
• Locus tag: ECIAI39_RS10475
• Protein ID: WP_001110446.1
• Coordinates: 2125648..2125941 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECIAI39_RS10465	2120797..2121756	-	960	WP_000846343.1	23S rRNA pseudouridine(955/2504/2580) synthase RluC	-
ECIAI39_RS10470	2122329..2125514	+	3186	WP_000827392.1	ribonuclease E	-
ECIAI39_RS10475	2125648..2125941	+	294	WP_001110446.1	DUF1778 domain-containing protein	Antitoxin
ECIAI39_RS10480	2125938..2126432	+	495	WP_000613629.1	GNAT family N-acetyltransferase	Toxin
ECIAI39_RS10485	2126527..2127480	-	954	WP_001212748.1	flagellar hook-associated protein FlgL	-
ECIAI39_RS10490	2127492..2129135	-	1644	WP_000096449.1	flagellar hook-associated protein FlgK	-
ECIAI39_RS10495	2129201..2130142	-	942	WP_012602385.1	flagellar assembly peptidoglycan hydrolase FlgJ	-
ECIAI39_RS10500	2130142..2131239	-	1098	Protein_2061	flagellar basal body P-ring protein FlgI	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (81-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 18112.05 Da
• Isoelectric Point: 8.2617

>T23431 WP_000613629.1 NC_011750:2125938-2126432 [Escherichia coli IAI39]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRVLKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Nucleotide

• Length: 495 bp

>T23431 NC_011750:2125938-2126432 [Escherichia coli IAI39]
ATGATTCAGGCCCCCGAGCCACTCATGGCAAGGCATCAATTCACCTCTTTTTGCTCGGGAGTAGAAACGATGGATAACTG
GCTAAAGCAGAGAGTCCTAAAAAATCAGCTTGCCGGCGCATCACGTACATTTGTTAGCTGTGATACGTATAGCAACGTTT
TGGCATATTATTCGCTTGCTTCCAGCGCCGTTGAAACGTATGTCGCGACAGGACGATTTCGCCGAAATATGCCAGAACCG
ATCCCTGTTGTAGTACTTGGTCGTCTGGCAATAGATAAATCATTACAGGGTCAGGGCATCGGTCGTGCGATGGTCAGAGA
TGCCGGTCTACGAGTACTACAGGCGGCTGAAGTCATTGGTATTCGCGGAATGTTGGTCCATGCGTTATCAGACCAGGCCC
GTGAGTTTTATCTGCGAGTGGGGTTTGAACCATCGCCTGTCGATTCTATGATACTGATGGCAACATTGGCTGATTTGCAG
GAATGTCTTAAATAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11079.67 Da
• Isoelectric Point: 10.5423

>AT23431 WP_001110446.1 NC_011750:2125648-2125941 [Escherichia coli IAI39]
MPAANNIPARRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT23431 NC_011750:2125648-2125941 [Escherichia coli IAI39]
ATGCCAGCAGCCAATAACATTCCAGCCAGGCGCGAATCTTTAAACTTGCGCATAAAACCCGCAGAACGCGATCTTATTGA
CCGGGCCGCTAAAGCCCGAGGAAAAAATCGCACCGAATTTGTCCTTGAGGCCGCCAGAGCCGCAGCTGAAGAGGCGCTCA
TTGAGCAGAGAATTTTCAGGATCGATTCTGATGATTACCAGCTATTTTTGAATCGTCTGGATCAGCCCCCCGCGCCCAAT
GCTGCATTACGCAAAACCATGCAAACACCCGCGCCGTGGGAGCAAAAAAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A829G775