Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1129802..1130456 Replicon chromosome
Accession NZ_CP091659
Organism Escherichia coli strain KFu024

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag L6L61_RS06295 Protein ID WP_000244772.1
Coordinates 1129802..1130209 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag L6L61_RS06300 Protein ID WP_000354046.1
Coordinates 1130190..1130456 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6L61_RS06275 (1125759) 1125759..1127492 - 1734 WP_113396745.1 single-stranded-DNA-specific exonuclease RecJ -
L6L61_RS06280 (1127498) 1127498..1128208 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
L6L61_RS06285 (1128233) 1128233..1129129 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
L6L61_RS06290 (1129241) 1129241..1129762 + 522 WP_001055874.1 flavodoxin FldB -
L6L61_RS06295 (1129802) 1129802..1130209 - 408 WP_000244772.1 protein YgfX Toxin
L6L61_RS06300 (1130190) 1130190..1130456 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
L6L61_RS06305 (1130699) 1130699..1131679 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
L6L61_RS06310 (1131932) 1131932..1132912 + 981 WP_000399648.1 IS110-like element IS621 family transposase -
L6L61_RS06315 (1133035) 1133035..1133694 - 660 WP_000250274.1 hemolysin III family protein -
L6L61_RS06320 (1133858) 1133858..1134169 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 1131932..1132912 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T234304 WP_000244772.1 NZ_CP091659:c1130209-1129802 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T234304 NZ_LR130513:c2218875-2218771 [Staphylococcus aureus]
TTGTTACTCCTAGAAAGGACTAGCATGTCTGATTTTGAAATGCTGATGGTTGTATTAACAATCATTGGTTTAGTATTGAT
TAGTAATCAAGGCCATAAAAAATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT234304 WP_000354046.1 NZ_CP091659:c1130456-1130190 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT234304 NZ_LR130513:2218673-2218715 [Staphylococcus aureus]
CAGAAACATGTTACCCTAATGAGCCCGTTAAAAAGACGGTGAC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References