Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 122092..122617 Replicon plasmid pKFu024_1
Accession NZ_CP091658
Organism Escherichia coli strain KFu024

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag L6L61_RS00800 Protein ID WP_001159871.1
Coordinates 122092..122397 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag L6L61_RS00805 Protein ID WP_000813630.1
Coordinates 122399..122617 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6L61_RS00785 (118054) 118054..119220 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
L6L61_RS00790 (119808) 119808..120563 - 756 WP_000852146.1 replication initiation protein RepE -
L6L61_RS00795 (121285) 121285..122091 - 807 WP_000016970.1 site-specific integrase -
L6L61_RS00800 (122092) 122092..122397 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
L6L61_RS00805 (122399) 122399..122617 - 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
L6L61_RS00810 (123177) 123177..123407 + 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin -
L6L61_RS00815 (123404) 123404..123820 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -
L6L61_RS00820 (123895) 123895..125460 + 1566 WP_001128474.1 AAA family ATPase -
L6L61_RS00825 (125445) 125445..126467 + 1023 WP_000361402.1 helicase UvrD -
L6L61_RS24975 (126721) 126721..127110 - 390 WP_071999143.1 IS1 family transposase -
L6L61_RS24980 (127132) 127132..127407 - 276 WP_000179213.1 IS1-like element transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD / aac(3)-IId / erm(B) / mph(A) / sul1 / qacE / aadA5 iutA / iucD / iucC / iucB / iucA 1..137247 137247
- flank IS/Tn - - 126721..127068 347


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T234300 WP_001159871.1 NZ_CP091658:c122397-122092 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T234300 NZ_LR130513:c1926442-1926290 [Staphylococcus aureus]
ATGTCTAAAGACAAAGATCCAAAATTAAATTATCATGAAGAAGAAAACAGTATGGTAACTGATTTTGAAGATTTAAAAGA
ATTAAGTAAAGAAATGGAACAAATTTCCGATCAAAATGATCAAGAAAAAAATTCTGAAGAAGACAGTCAGTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT234300 WP_000813630.1 NZ_CP091658:c122617-122399 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT234300 NZ_LR130513:c1927066-1926467 [Staphylococcus aureus]
ATGGCAATGAACTTTAAAGTCTTTGACAATAGTCAACTTGTAGCAGAATATGCTGCTGATATTATTAGAAAGCAATTTAA
CAATAATCCTACTACAATTGCAGGTTTTCATTTAGATACAGATCAAGCGCCAGTTCTAGATGAATTAAAGAAAAATGTTG
AAAAACATGCTGTTGATTTTAGCCAAATAAATATTTTAGATTATGACGATAAAAAATCATATTTCGAAGCGTTAGGTGTA
CCAGCAGGTCAAGTTTATCCAATTGCTTATGAAAAAGATGCAATCGAATTAATCGCTGATAAGATTAAAACTAAAGAAAA
TAAAGGGAAATTAACATTACAAGTTGTTTCTATCGATGAGCAAGGTAAGTTAAATGTTAGTATTCGTCAAGGACTAATGG
AAGCAAGAGAAATTTTCTTAGTAGTGACAGGTGCTAATAAACGAGATGTAGTTGAAAAATTATATCAAGAAAATGGTAAA
ACAAGCTTCGAACCAGCCGATTTAAAAGCACATAGAATGGTAAATGTTATTCTTGATAAAGAAGCGGCTGCAGGTTTACC
TGAAGATGTTAAAGCTTACTTTACGTCACGCTTTGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References