Detailed information of TA system TA234280

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 72838..73397
• Replicon: plasmid pKMi025_2
• Accession: NZ_CP091653
• Organism: Escherichia coli strain KMi025

Toxin (Protein)

• Gene name: relE
• Uniprot ID: U9YB49
• Locus tag: L6L63_RS23705
• Protein ID: WP_000421259.1
• Coordinates: 73122..73397 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: U9Y625
• Locus tag: L6L63_RS23700
• Protein ID: WP_001178087.1
• Coordinates: 72838..73122 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L6L63_RS23680 (68487)	68487..69020	-	534	WP_001372168.1	transcription termination/antitermination NusG family protein	-
L6L63_RS23685 (69462)	69462..69749	-	288	WP_000074849.1	conjugal transfer protein TraA	-
L6L63_RS23690 (69667)	69667..69897	-	231	WP_228772074.1	ash family protein	-
L6L63_RS23695 (70903)	70903..71934	+	1032	WP_000907852.1	plasmid replication initiator RepA	-
L6L63_RS23700 (72838)	72838..73122	+	285	WP_001178087.1	ribbon-helix-helix domain-containing protein	Antitoxin
L6L63_RS23705 (73122)	73122..73397	+	276	WP_000421259.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
L6L63_RS23710 (73503)	73503..73706	+	204	Protein_74	hypothetical protein	-
L6L63_RS23715 (73976)	73976..74242	-	267	WP_000627832.1	hypothetical protein	-
L6L63_RS23720 (74242)	74242..74736	-	495	WP_015058884.1	hypothetical protein	-
L6L63_RS23725 (74792)	74792..75394	-	603	WP_061671620.1	ProQ/FINO family protein	-
L6L63_RS23730 (75748)	75748..77094	+	1347	WP_001132027.1	protein YagA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-8	-	1..113312	113312

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10736.45 Da
• Isoelectric Point: 9.9324

>T234280 WP_000421259.1 NZ_CP091653:73122-73397 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELTGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T234280 NZ_CP091653:73122-73397 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
GGCCGCCAGAACGGTGCAGTCCCTGACACAGGCTCCGGTCATTCTGTTAACTCATCCACGTATGGGGGAACAGTTATTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTACTGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10488.92 Da
• Isoelectric Point: 5.6558

>AT234280 WP_001178087.1 NZ_CP091653:72838-73122 [Escherichia coli]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWIIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT234280 NZ_CP091653:72838-73122 [Escherichia coli]
ATGCAAATGAAAAACAATACCGCACAGGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGATTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: U9YB49

Antitoxin

• AlphaFold DB: U9Y625