234264

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 3300219..3300795 Replicon chromosome
Accession NZ_CP091652
Organism Escherichia coli strain KMi025

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag L6L63_RS16005 Protein ID WP_001297643.1
Coordinates 3300219..3300506 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag L6L63_RS16010 Protein ID WP_000063156.1
Coordinates 3300493..3300795 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6L63_RS15985 (3296472) 3296472..3297752 - 1281 WP_001037380.1 DUF445 domain-containing protein -
L6L63_RS15990 (3297793) 3297793..3299025 - 1233 WP_001380906.1 multidrug efflux MFS transporter MdtM -
L6L63_RS15995 (3299171) 3299171..3299266 - 96 Protein_3120 dienelactone hydrolase family protein -
L6L63_RS16000 (3299323) 3299323..3300020 + 698 Protein_3121 IS1 family transposase -
L6L63_RS16005 (3300219) 3300219..3300506 + 288 WP_001297643.1 BrnT family toxin Toxin
L6L63_RS16010 (3300493) 3300493..3300795 + 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
L6L63_RS16015 (3300829) 3300829..3301785 - 957 WP_001297640.1 GTPase -
L6L63_RS16020 (3301796) 3301796..3301999 - 204 WP_000467859.1 YbdD/YjiX family protein -
L6L63_RS16025 (3302049) 3302049..3304199 - 2151 WP_001295524.1 pyruvate/proton symporter BtsT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - cheD 3299517..3367380 67863


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T234264 WP_001297643.1 NZ_CP091652:3300219-3300506 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T234264 NZ_LR129841:c1654445-1654266 [Streptococcus pneumoniae]
ATGCCTATGACACAAAAAGAGATGGTTAAACTCCTTACGGCCCATGGTTGGATAAAGACTAGAGGCGGTAAAGGCTCCCA
TATTAAAATGGAAAAGCAAGGGGAAAGACCTATCACAATCCTTCATGGTAAGCTGAATAAGTACACTGAAAGAGGGATAA
GAAAGCAAGCTGGGTTGTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT234264 WP_000063156.1 NZ_CP091652:3300493-3300795 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT234264 NZ_LR129841:c1654229-1653777 [Streptococcus pneumoniae]
ATGTTAGTTACGTATCCAGCTTTATTTTATTATGACGACACAGACGGAACAGAAGCGACTTATTTTGTCCATTTCCCAGA
TTTTGAATACTCAGCTACACAAGGAGAGGGGATTTCTGAGGCTTTGGCTATGGGGTCGGAGTGGCTAGGGATAACTGTTG
CAGATTTGATAGAGAGTGATGGAGAGTTACCTCAGCCATCAGATATCAATAGCTTGTCTTTAATTGATAATGATCCTTTT
AAAGATGATGAAGATTTCGTGTCAACCTATGACCTTGATAAATCTTTTATTTCTATGGTATCTGTTGATGTATCAGAATA
CCTAGGAAGTCAGGAACCCATTAAAAAGACTTTGACCATACCAAAATGGGCAGATAAGTTGGGACGAGAAATGGGACTTA
ACTTTTCTCAGACTTTGACAGACGCTATTGCAGATAAGAAAGTTCAAGCCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References