Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 3069500..3069997 Replicon chromosome
Accession NZ_CP091652
Organism Escherichia coli strain KMi025

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag L6L63_RS14830 Protein ID WP_001173343.1
Coordinates 3069824..3069997 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag L6L63_RS14825 Protein ID WP_000398619.1
Coordinates 3069500..3069796 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6L63_RS14805 (3066231) 3066231..3066950 - 720 WP_000611288.1 two-component system response regulator DcuR -
L6L63_RS14810 (3066947) 3066947..3068578 - 1632 WP_001216477.1 sensor histidine kinase -
L6L63_RS14815 (3068759) 3068759..3068989 + 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
L6L63_RS14820 (3069001) 3069001..3069273 + 273 WP_000405651.1 GNAT family N-acetyltransferase -
L6L63_RS14825 (3069500) 3069500..3069796 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
L6L63_RS14830 (3069824) 3069824..3069997 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
L6L63_RS14835 (3070116) 3070116..3071633 - 1518 WP_001295074.1 lysine--tRNA ligase -
L6L63_RS14840 (3071870) 3071870..3073327 - 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T234263 WP_001173343.1 NZ_CP091652:3069824-3069997 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T234263 NZ_LR129841:c1605477-1605223 [Streptococcus pneumoniae]
ATGCTGCTCAAGTTTACAGAAGATGCCTGGGCAGATTATTGCTACTGGCAAAATCAGGATAAGAAAACGTTAAAAAGAAT
CAATAAACTAATCAAGGATATTCAACGTGATCCCTTTACAGGAATAGGTAAACCAGAACCACTCAAATATGATTACCAAG
GAGCCTGGTCACGGCGTATTGATGCAGAAAATCGCTTGATTTATATGATGGATGGAGATAGCGTGGCTTTCTTGTCCTTT
AAAGATCATTACTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT234263 WP_000398619.1 NZ_CP091652:3069500-3069796 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT234263 NZ_LR129841:c1605735-1605481 [Streptococcus pneumoniae]
ATGGAAGCAGTCCTTTACTCAACATTCCGAAATCATTTAAAGGACTACATGAAGAAGGTAAATGATGAATTTGAGCCTTT
GACGGTGGTCAATAAAAATCCAGATGAGGACATTGTAGTCCTTTCAAAGAGTGAGTGGGATAGTATCCAAGAAACCCTGA
GAATTGCTCAAAATAAGGAACTTTCTGATAAGGTTTTGCGAGGAATGGCTCAAGTTCGTGCTGGAAGTACTCAGGTCCAT
GTTATTGAGGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References