Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1693202..1693856 Replicon chromosome
Accession NZ_CP091652
Organism Escherichia coli strain KMi025

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag L6L63_RS08235 Protein ID WP_000244777.1
Coordinates 1693202..1693609 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag L6L63_RS08240 Protein ID WP_000354046.1
Coordinates 1693590..1693856 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6L63_RS08215 (1689159) 1689159..1690892 - 1734 WP_000813201.1 single-stranded-DNA-specific exonuclease RecJ -
L6L63_RS08220 (1690898) 1690898..1691608 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
L6L63_RS08225 (1691633) 1691633..1692529 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
L6L63_RS08230 (1692641) 1692641..1693162 + 522 WP_001055874.1 flavodoxin FldB -
L6L63_RS08235 (1693202) 1693202..1693609 - 408 WP_000244777.1 protein YgfX Toxin
L6L63_RS08240 (1693590) 1693590..1693856 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
L6L63_RS08245 (1694099) 1694099..1695079 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
L6L63_RS08250 (1695275) 1695275..1695934 - 660 WP_000250274.1 hemolysin III family protein -
L6L63_RS08255 (1696098) 1696098..1696409 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
L6L63_RS08260 (1696454) 1696454..1697887 + 1434 WP_001338826.1 6-phospho-beta-glucosidase BglA -
L6L63_RS08265 (1697944) 1697944..1698687 - 744 WP_000951962.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T234258 WP_000244777.1 NZ_CP091652:c1693609-1693202 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T234258 NZ_LR129840:c1068548-1067778 [Streptococcus pneumoniae]
GTGGAAATCCAAGATTATACTGACAGTGAATTCAAACATGCTCTAGCACGGAATCTTCGTTCACTGACAAGAGGAAAAAA
GTCCAGTAAGCAACCTATAGCGATTTTGCTCGGAGGGCAAAGTGGTGCCGGTAAGACTACAATTCATCGTATTAAACAGA
AAGAATTTCAAGGAAATATTGTTATCATAGATGGAGATAGTTTTCGTTCTCAGCATCCACACTATTTAGAACTTCAGCAA
GAATATGGCAAAGACAGTGTTGAATACACCAAATATTTTGCAGGTAAAATGGTAGAGTCTTTAGTAAGAGAATTGAGTCA
TTTGGGATACAATCTTTTGATAGAGGGAACTTTACGAACTATTGATGTTCCAAAGAAAACAGCACAACTCTTGAAAAGTA
GGGGATATGAAATACAATTAGCCTTGATTGCGACAAAGCCTAAGCTGTCCTATCTGAGCACCCTTATCCGATACGAAGAA
CTGTACGCTATTAACCCAAATCAAGCACGCGCAACTCCAAAAGAACATCATGATTTCATTGTAAATCATCTAGTTGATAA
TACACGACAATTGGAAGAACTAGCTATCTTTGAAAGAATTCAAATTTACCAACGAGATAGAGGTTGTGTATATGATGCAA
AAGAAAATACAACTTCAGCGGCAGATGTTCTTCAAGAGTTACTGTTTGGGGAGTGGAGTCAAGTAGAGAAGGATATGCTT
AAATCTGGAGAAGAAAGATTGAAAGATTTAACTAATCGAAATGGTTGTTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT234258 WP_000354046.1 NZ_CP091652:c1693856-1693590 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT234258 NZ_LR129840:c1069024-1068548 [Streptococcus pneumoniae]
ATGATTGGAAAGAACATAAAATCCTTACGTAAAACACATGAATTAACACAACCCGAATTTGCCCGAATTATAGGAATTTC
TCGAAATAGCTTGAGTCGTTATGAAAATGGAACGAGTTCAGTCTCTACCGAATTAATAGACATCATTTGTCAGAAGTTTA
ATGTATCTTATGTCGATATTGTAGGAGAAGATAAAATGCTCAATCCTGTTGAAGACTATGAATTGACTTTAAAAATTGAA
ATTGTGAAAGAAAGAGGTGCTAATCTTTTATCTCGACTCTATCGTTATCAAGATAGTCAGGGAATTAGCATTGATGATGA
ATCTAATCCTTGGATTTTAATGAGTGATGATCTATCTGACTTGATTCATACGAATATCTATTTAGTAGAAACTTTTGATG
AAATAGAGAGATATAGCGGCTATTTGGATGGAATTGAACGTATGTTAGAGATATCTGAAAAACGGATGGTAGCCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References