Detailed information of TA system TA234257

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 1558350..1558933
• Replicon: chromosome
• Accession: NZ_CP091652
• Organism: Escherichia coli strain KMi025

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: L6L63_RS07630
• Protein ID: WP_049253269.1
• Coordinates: 1558350..1558685 (-)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A1V2T5M9
• Locus tag: L6L63_RS07635
• Protein ID: WP_000581941.1
• Coordinates: 1558685..1558933 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L6L63_RS07615 (1554237)	1554237..1555535	-	1299	WP_000036723.1	phosphopyruvate hydratase	-
L6L63_RS07620 (1555623)	1555623..1557260	-	1638	WP_000210878.1	CTP synthase (glutamine hydrolyzing)	-
L6L63_RS07625 (1557488)	1557488..1558279	-	792	WP_001071648.1	nucleoside triphosphate pyrophosphohydrolase	-
L6L63_RS07630 (1558350)	1558350..1558685	-	336	WP_049253269.1	endoribonuclease MazF	Toxin
L6L63_RS07635 (1558685)	1558685..1558933	-	249	WP_000581941.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
L6L63_RS07640 (1559011)	1559011..1561245	-	2235	WP_000226815.1	GTP pyrophosphokinase	-
L6L63_RS07645 (1561293)	1561293..1562594	-	1302	WP_023281448.1	23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12197.18 Da
• Isoelectric Point: 8.7218

>T234257 WP_049253269.1 NZ_CP091652:c1558685-1558350 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQARHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Nucleotide

• Length: 336 bp

>T234257 NZ_LR129840:977866-978627 [Streptococcus pneumoniae]
ATGGAAATCCAAGATTATACTGATAGTGAATTCAAACATGCTTTAGCAAGGAATCTTCGTTCACTGACAAGAGGAAAAAA
GTCCAGTAAACAACCTATAGCGATTTTGCTTGGAGGTCAAAGTGGTGCCGGTAAGACTACAATTCATCGTATTAAACAGA
AAGAATTTCAAGGAAATATTGTTATCATAGATGGTGATAGTTTTCGTTCTCAGCATCCACACTATTTAGAACTGCAGCAA
GAATATGGCAAAGACAGTGTAGAATACACCAAAGATTTTGCAGGTAAAATGGTAGAGTCTTTAGTAACAGAATTGAGTCA
TTTGGGATACAATCTTTTGATAGAGGGAACTTTACGAACGATTGACGTTCCAAAGGAAACAGCACAACTCTTGAAAAGTA
AGGGATATGAAGTACAATTAGCCTTGATTGCGACAAAGCCTAAGCTGTCCTATCTGAGCACCCTTATCCGATACGAAGAA
CTGTACGCTATCAATCCAAATCAAGCACGCGCAACTCCAAAAGAACATCATGATTTCATTGTAAATCATCTAGTTGATAA
TACACGACAACTGGAAGAACTGGCTATCTTTGAAAGAATTCAAATTTACCAACGAGATAGAAGTTGTGTATATGATTCAA
AAGAAAATACAACTTCAGCAGCAGATGTTCTTCAAGAGTTACTGTTTGGAGAGTGGAGTCAGGTAGAAAAGGAGATGTTG
AGGGTAGGGGAAAAGAGACTTAATGAATTACTTGAAAAATAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9385.76 Da
• Isoelectric Point: 4.4665

>AT234257 WP_000581941.1 NZ_CP091652:c1558933-1558685 [Escherichia coli]
MIHSSVKRWGNSPAVRIPTTLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Nucleotide

• Length: 249 bp

>AT234257 NZ_LR129840:977390-977866 [Streptococcus pneumoniae]
ATGATTGGAAAGAACATAAAATCCTTACGTAAAACACATGACTTAACACAACCCGAATTTGCACGAATTATAGGAATTTC
TCGAAATAGCTTGAGTCGTTATGAAAATGGAACGAGTTCAGTCTCTACGGAATTAATTGACATCATTTGTCAGAAGTTTA
ATGTATCTTATGTCGATATTGTAGGAGAAGATAAAATGCTCAATCCTGTTGAAGATTATGAATTGACTTTGAAAATTGAA
ATTGTGAAAGAAAGAGGTGCTAATCTATTATCTCGACTCTATCGTTATCAAGATAGTCAGGGAATTAGCATTGATGATGA
ATCTAATCCTTGGATTTTAATGAGTGATGATCTATCTGACTTGATTCATACGAATATCTATTTAGTAGAAACTTTTGATG
AAATAGAGAGATATAGTGGCTATTTGGATGGAATTGAACGTATGTTAGAGATATCTGAAAAGCGGATGGTAGCTTAA

Structures

Antitoxin

• AlphaFold DB: A0A1V2T5M9