234252

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 147012..147538 Replicon chromosome
Accession NZ_CP091652
Organism Escherichia coli strain KMi025

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag L6L63_RS00735 Protein ID WP_000323025.1
Coordinates 147251..147538 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag L6L63_RS00730 Protein ID WP_000534858.1
Coordinates 147012..147251 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6L63_RS00695 (142740) 142740..142970 - 231 Protein_135 IS66 family insertion sequence element accessory protein TnpB -
L6L63_RS00700 (142976) 142976..143098 - 123 Protein_136 transposase -
L6L63_RS00705 (143341) 143341..143762 + 422 Protein_137 hypothetical protein -
L6L63_RS00710 (144418) 144418..145440 + 1023 Protein_138 ISNCY family transposase -
L6L63_RS00715 (145561) 145561..145710 + 150 WP_011443592.1 protein YdfW -
L6L63_RS00720 (146147) 146147..146479 - 333 WP_001301033.1 FlxA-like family protein -
L6L63_RS00725 (146682) 146682..146987 - 306 WP_001326990.1 protein YdfV -
L6L63_RS00730 (147012) 147012..147251 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
L6L63_RS00735 (147251) 147251..147538 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
L6L63_RS00740 (147610) 147610..147765 + 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
L6L63_RS00745 (147982) 147982..148233 + 252 WP_000980994.1 protein Rem -
L6L63_RS00750 (148300) 148300..148578 + 279 WP_012304870.1 hypothetical protein -
L6L63_RS00755 (148580) 148580..149629 + 1050 WP_001265199.1 DUF968 domain-containing protein -
L6L63_RS00760 (149643) 149643..150395 + 753 WP_001047135.1 antitermination protein -
L6L63_RS00765 (150673) 150673..150762 - 90 WP_120795389.1 hypothetical protein -
L6L63_RS00770 (150817) 150817..151029 - 213 WP_000087756.1 cold shock-like protein CspF -
L6L63_RS00775 (151330) 151330..151545 + 216 WP_000066484.1 cold shock-like protein CspB -
L6L63_RS00780 (151909) 151909..152079 - 171 WP_211180497.1 putative zinc-binding protein YnfU -
L6L63_RS00785 (152299) 152299..152514 + 216 WP_000839590.1 phage lysis protein EssD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 132191..178923 46732


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T234252 WP_000323025.1 NZ_CP091652:147251-147538 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T234252 NZ_LR027878:c2424257-2424153 [Staphylococcus aureus]
TTGTTACTCCTAGAAAGGACTAGCATGTCTGATTTTGAAATGCTTATGGTTGTATTAACAATCATTGGTTTAGTATTGAT
TAGTACTCAAGACCATAAAAAATAA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT234252 WP_000534858.1 NZ_CP091652:147012-147251 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT234252 NZ_LR027878:2424040-2424095 [Staphylococcus aureus]
AAAAAGGGCAACACTCGGAAACATGTTACCCTAATGAGCCCGTTAAAAAGACGGTG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
AlphaFold DB A0A4V1CTS8

References