Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4660816..4661313 Replicon chromosome
Accession NZ_CP091651
Organism Escherichia coli strain KTa008

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag L6L70_RS22575 Protein ID WP_001173343.1
Coordinates 4660816..4660989 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag L6L70_RS22580 Protein ID WP_000398619.1
Coordinates 4661017..4661313 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6L70_RS22565 (4657486) 4657486..4658943 + 1458 WP_001550473.1 dipeptide/tripeptide permease DtpC -
L6L70_RS22570 (4659180) 4659180..4660697 + 1518 WP_001550472.1 lysine--tRNA ligase -
L6L70_RS22575 (4660816) 4660816..4660989 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
L6L70_RS22580 (4661017) 4661017..4661313 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
L6L70_RS22585 (4661542) 4661542..4661814 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
L6L70_RS22590 (4661826) 4661826..4662056 - 231 WP_001550470.1 4Fe-4S mono-cluster protein YjdI -
L6L70_RS22595 (4662236) 4662236..4663867 + 1632 WP_001216477.1 sensor histidine kinase -
L6L70_RS22600 (4663864) 4663864..4664583 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T234250 WP_001173343.1 NZ_CP091651:c4660989-4660816 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T234250 NZ_LR027878:c2347290-2346928 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT234250 WP_000398619.1 NZ_CP091651:c4661313-4661017 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT234250 NZ_LR027878:c2347457-2347287 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References