Detailed information of TA system TA234243

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 3120106..3120890
• Replicon: chromosome
• Accession: NZ_CP091651
• Organism: Escherichia coli strain KTa008

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: V0T0H9
• Locus tag: L6L70_RS15240
• Protein ID: WP_000613626.1
• Coordinates: 3120396..3120890 (+)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: L4JCW6
• Locus tag: L6L70_RS15235
• Protein ID: WP_001110447.1
• Coordinates: 3120106..3120399 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L6L70_RS15225 (3115256)	3115256..3116215	-	960	WP_000846342.1	23S rRNA pseudouridine(955/2504/2580) synthase RluC	-
L6L70_RS15230 (3116788)	3116788..3119973	+	3186	WP_001550951.1	ribonuclease E	-
L6L70_RS15235 (3120106)	3120106..3120399	+	294	WP_001110447.1	DUF1778 domain-containing protein	Antitoxin
L6L70_RS15240 (3120396)	3120396..3120890	+	495	WP_000613626.1	GNAT family N-acetyltransferase	Toxin
L6L70_RS15245 (3120985)	3120985..3121938	-	954	WP_001212768.1	flagellar hook-associated protein FlgL	-
L6L70_RS15250 (3121950)	3121950..3123593	-	1644	WP_000096478.1	flagellar hook-associated protein FlgK	-
L6L70_RS15255 (3123659)	3123659..3124600	-	942	WP_001366226.1	flagellar assembly peptidoglycan hydrolase FlgJ	-
L6L70_RS15260 (3124600)	3124600..3125697	-	1098	WP_134259463.1	flagellar basal body P-ring protein FlgI	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (81-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 18084.00 Da
• Isoelectric Point: 8.2617

>T234243 WP_000613626.1 NZ_CP091651:3120396-3120890 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Nucleotide

• Length: 495 bp

>T234243 NZ_LR027877:c2630102-2629836 [Staphylococcus aureus]
ATGAGCAATTACACGGTTAAGATTAAAAATTCAGCGAAATCAGATTTAAAGAAAATAAAACATTCTTATTTAAAGAAGTC
ATTTTTAGAAATTGTTGAGACTTTAAAAAATGATCCGTATAAAATAACACAATCTTTTGAAAAATTAGAGCCTAAATATT
TAGAGCGATATTCAAGAAGAATTAACCATCAGCACAGGGTCGTCTATACCGTAGATGATCGAAATAAAGAAGTATTAATA
CTATCGGCATGGTCACATTATGATTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11109.69 Da
• Isoelectric Point: 10.5423

>AT234243 WP_001110447.1 NZ_CP091651:3120106-3120399 [Escherichia coli]
MPAANNIPTRRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT234243 NZ_LR027877:c2630359-2630102 [Staphylococcus aureus]
ATGATTATCACTAGCCCTACAGAAGCGAGAAAAGATTTTTATCAATTACTAAAAAATGTTAATAATAATCACGAACCAAT
TTATATTAGTGGCAATAATGCCGAAAATAATGCTGTGATTATAGGTTTAGAAGATTGGAAAAGTATACAAGAGACAATAT
ATCTTGAATCTACTGGTACAATGGACAAAGTAAGAGAAAGAGAAAAAGATAATAGTGGTACAACAAATATAGATGATATT
GATTGGGATAATCTTTAA

Structures

Toxin

• AlphaFold DB: V0T0H9

Antitoxin

• AlphaFold DB: L4JCW6