Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 843458..844293 Replicon chromosome
Accession NZ_CP091651
Organism Escherichia coli strain KTa008

Toxin (Protein)


Gene name yeeV Uniprot ID A0A0A1A9A5
Locus tag L6L70_RS04030 Protein ID WP_001564063.1
Coordinates 843458..843835 (-) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A0K5N986
Locus tag L6L70_RS04035 Protein ID WP_021553056.1
Coordinates 843925..844293 (-) Length 123 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6L70_RS04000 (838863) 838863..839270 - 408 Protein_783 IS5-like element IS903B family transposase -
L6L70_RS04005 (839350) 839350..840225 - 876 WP_001617865.1 extended-spectrum class A beta-lactamase CTX-M-14 -
L6L70_RS04010 (840475) 840475..841737 - 1263 WP_000608644.1 IS1380-like element ISEcp1 family transposase -
L6L70_RS04015 (842112) 842112..842954 - 843 Protein_786 DUF4942 domain-containing protein -
L6L70_RS04020 (843039) 843039..843233 - 195 WP_024181941.1 DUF957 domain-containing protein -
L6L70_RS04025 (843312) 843312..843461 - 150 Protein_788 DUF5983 family protein -
L6L70_RS04030 (843458) 843458..843835 - 378 WP_001564063.1 TA system toxin CbtA family protein Toxin
L6L70_RS04035 (843925) 843925..844293 - 369 WP_021553056.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
L6L70_RS04040 (844456) 844456..844677 - 222 WP_000692345.1 DUF987 domain-containing protein -
L6L70_RS04045 (844742) 844742..845218 - 477 WP_021553055.1 RadC family protein -
L6L70_RS04050 (845234) 845234..845713 - 480 WP_001564060.1 antirestriction protein -
L6L70_RS04055 (845979) 845979..846797 - 819 WP_001564059.1 DUF932 domain-containing protein -
L6L70_RS04060 (846887) 846887..847120 - 234 WP_001278293.1 DUF905 family protein -
L6L70_RS04065 (847126) 847126..847803 - 678 WP_001564058.1 hypothetical protein -
L6L70_RS04070 (847954) 847954..848634 - 681 WP_001278649.1 WYL domain-containing protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - papX 841985..915589 73604
- flank IS/Tn blaCTX-M-14 - 839350..841737 2387


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(1-112)

Antitoxin

(18-117)


Sequences


Toxin        


Download         Length: 126 a.a.        Molecular weight: 14094.09 Da        Isoelectric Point: 7.8045

>T234227 WP_001564063.1 NZ_CP091651:c843835-843458 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYSLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYVLVRTDQPGF
SAGASSQLINSIDILRARRATGLMTRSNYRTVNNITRGKHPEAKQ

Download         Length: 378 bp

>T234227 NZ_LR027876:c2243558-2243349 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGATGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13615.39 Da        Isoelectric Point: 7.0264

>AT234227 WP_021553056.1 NZ_CP091651:c844293-843925 [Escherichia coli]
VSDTLSGTTHPDDNHDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGNRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Download         Length: 369 bp

>AT234227 NZ_LR027876:c2243863-2243570 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A0A1A9A5


Antitoxin

Source ID Structure
AlphaFold DB A0A0K5N986

References