Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 2713126..2713751 Replicon chromosome
Accession NZ_CP091650
Organism Klebsiella pneumoniae strain TAKPN-1

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag L6D56_RS13105 Protein ID WP_002882817.1
Coordinates 2713126..2713509 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag L6D56_RS13110 Protein ID WP_004150355.1
Coordinates 2713509..2713751 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6D56_RS13090 2710492..2711394 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
L6D56_RS13095 2711391..2712026 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
L6D56_RS13100 2712023..2712952 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
L6D56_RS13105 2713126..2713509 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
L6D56_RS13110 2713509..2713751 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
L6D56_RS13115 2713956..2714873 + 918 WP_004901674.1 alpha/beta hydrolase -
L6D56_RS13120 2714887..2715828 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
L6D56_RS13125 2715873..2716310 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
L6D56_RS13130 2716307..2717167 - 861 WP_004146232.1 virulence factor BrkB family protein -
L6D56_RS13135 2717161..2717760 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T234212 WP_002882817.1 NZ_CP091650:c2713509-2713126 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T234212 NZ_LR027874:c1970025-1969873 [Staphylococcus aureus]
ATGTCTAAAGACAAAGATCCAAAATTAAATTATCATGAAGAAGAAAACAGTATGGTAACGGATTTTGAAGATTTAAAAGA
ATTAGGTAAAGAAATGGAACAAATCTCTGATCAAAATGATCAAGAAAAGAATTCTGAAGAAGACAGTCAGTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT234212 WP_004150355.1 NZ_CP091650:c2713751-2713509 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT234212 NZ_LR027874:c1970649-1970050 [Staphylococcus aureus]
ATGGCAATGAACTTTAAAGTCTTTGACAATAGTCAACTTGTAGCAGAATATGCTGCTGATATTATTAGAAAGCAATTTAA
CAATAATCCTACTACAATTGCAGGTTTTCATTTAGATACAGATCAAGCGCCAGTTCTAGATGAATTAAAGAAAAATGTTG
AAAAACATGCTGTTGATTTTAGCCAAATAAATATTTTAGATTATGACGATAAAAAATCATATTTCGAAGCGTTAGGTGTA
CCAGCAGGTCAAGTTTATCCAATTGCTTATGAAAAAGATGCAATCGAATTAATCGCTGATAAGATTAAAACTAAAGAAAA
TAAAGGGAAATTAACATTACAAGTTGTTTCTATCGATGAGCAAGGTAAGTTAAATGTTAGTATTCGTCAAGGACTAATGG
AAGCAAGAGAAATTTTCTTAGTAGTGACAGGTGCTAATAAACGAGATGTAGTTGAAAAATTATATCAAGAAAATGGTAAA
ACAAGCTTCGAACCAGCCGATTTAAAAGCACATAGAATGGTAAATGTTATTCTTGATAAAGAAGCGGCTGCAGGTTTACC
TGAAGATGTTAAAGCTTACTTTACGTCACGCTTTGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References