Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 2089824..2090634 Replicon chromosome
Accession NZ_CP091650
Organism Klebsiella pneumoniae strain TAKPN-1

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag L6D56_RS10185 Protein ID WP_004178461.1
Coordinates 2089824..2090357 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag L6D56_RS10190 Protein ID WP_002887278.1
Coordinates 2090368..2090634 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6D56_RS10180 2088655..2089776 + 1122 WP_021441121.1 cupin domain-containing protein -
L6D56_RS10185 2089824..2090357 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
L6D56_RS10190 2090368..2090634 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
L6D56_RS10195 2090737..2092170 - 1434 WP_021441120.1 Cu(+)/Ag(+) sensor histidine kinase -
L6D56_RS10200 2092160..2092843 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
L6D56_RS10205 2093016..2094401 + 1386 WP_038806759.1 efflux transporter outer membrane subunit -
L6D56_RS10210 2094419..2094763 + 345 WP_021441118.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T234208 WP_004178461.1 NZ_CP091650:c2090357-2089824 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T234208 NZ_LR027873:c2624964-2624698 [Staphylococcus aureus]
ATGAGCAATTACACGGTTAAGATTAAAAATTCAGCGAAATCAGATTTAAAGAAAATAAAACATTCTTATTTAAAGAAGTC
ATTTTTAGAAATTGTTGAGACTTTAAAAAATGATCCGTATAAAATAACACAATCTTTTGAAAAATTAGAGCCTAAATATT
TAGAGCGATATTCAAGAAGAATTAACCATCAGCACAGGGTCGTCTATACCGTAGATGATCGAAATAAAGAAGTATTAATA
CTATCGGCATGGTCACATTATGATTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT234208 WP_002887278.1 NZ_CP091650:c2090634-2090368 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT234208 NZ_LR027873:c2625221-2624964 [Staphylococcus aureus]
ATGATTATCACTAGCCCTACAGAAGCGAGAAAAGATTTTTATCAATTACTAAAAAATGTTAATAATAATCACGAACCAAT
TTATATTAGTGGCAATAATGCCGAAAATAATGCTGTGATTATAGGTTTAGAAGATTGGAAAAGTATACAAGAGACAATAT
ATCTTGAATCTACTGGTACAATGGACAAAGTAAGAGAAAGAGAAAAAGATAATAGTGGTACAACAAATATAGATGATATT
GATTGGGATAATCTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References