Detailed information of TA system TA234203

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 3440049..3440690
• Replicon: chromosome
• Accession: NZ_CP091647
• Organism: Burkholderia aenigmatica strain CMCC(B)23010

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: L3V59_RS33870
• Protein ID: WP_272931301.1
• Coordinates: 3440352..3440690 (-)
• Length: 113 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: U2GAF3
• Locus tag: L3V59_RS33865
• Protein ID: WP_021157290.1
• Coordinates: 3440049..3440348 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L3V59_RS33840 (L3V59_33840)	3435209..3436480	+	1272	WP_175017169.1	anthranilate 1,2-dioxygenase large subunit AndAc	-
L3V59_RS33845 (L3V59_33845)	3436498..3436983	+	486	WP_059521536.1	anthranilate 1,2-dioxygenase small subunit AndAd	-
L3V59_RS33850 (L3V59_33850)	3437004..3437330	+	327	WP_122949083.1	anthranilate 1,2-dioxygenase ferredoxin subunit AndAb	-
L3V59_RS33855 (L3V59_33855)	3437320..3438540	+	1221	WP_237434528.1	anthranilate 1,2-dioxygenase system ferredoxin--NAD(+) reductase	-
L3V59_RS33860 (L3V59_33860)	3438714..3440000	+	1287	WP_122949085.1	DUF445 domain-containing protein	-
L3V59_RS33865 (L3V59_33865)	3440049..3440348	-	300	WP_021157290.1	putative addiction module antidote protein	Antitoxin
L3V59_RS33870 (L3V59_33870)	3440352..3440690	-	339	WP_272931301.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
L3V59_RS33875 (L3V59_33875)	3441019..3442659	+	1641	WP_237434529.1	acetolactate synthase large subunit	-
L3V59_RS33880 (L3V59_33880)	3442688..3444121	+	1434	WP_122949088.1	aldehyde dehydrogenase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (26-98)

Antitoxin

• Uncharacterized protein HI1420 (6-93)

Sequences

Toxin

Protein

• Length: 113 a.a.
• Molecular weight: 12552.47 Da
• Isoelectric Point: 9.0539

>T234203 WP_272931301.1 NZ_CP091647:c3440690-3440352 [Burkholderia aenigmatica]
MVDTLQSMPYCAPTFSIRTTGVFDAWFAGLPDRVAKRRIQARIDRLSMGNPGDWKSAGSPVVEMRIDHGPGYRVYYVRRG
TIWVVLLCGGDKSTQQADIRAAHAMLAYLDME

Nucleotide

• Length: 339 bp

>T234203 NZ_LR027873:c2223423-2223061 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10633.01 Da
• Isoelectric Point: 6.3217

>AT234203 WP_021157290.1 NZ_CP091647:c3440348-3440049 [Burkholderia aenigmatica]
MDKISTRPWDSADHLKTEDDMADYFEACLQEAGDDPAFIAHALGVIARARGMSQVARDAGLSREGLYKALSHDGNPSFGT
ILKVIKALGLQLHGAKAHA

Nucleotide

• Length: 300 bp

>AT234203 NZ_LR027873:c2223590-2223420 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: U2GAF3