Detailed information of TA system TA023415

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 281450..282287
• Replicon: chromosome
• Accession: NC_011750
• Organism: Escherichia coli IAI39

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: V0SED1
• Locus tag: ECIAI39_RS01255
• Protein ID: WP_000227787.1
• Coordinates: 281745..282287 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: B1LJI1
• Locus tag: ECIAI39_RS01250
• Protein ID: WP_001353405.1
• Coordinates: 281450..281761 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECIAI39_RS01225	276470..277417	+	948	WP_001239436.1	cytochrome o ubiquinol oxidase subunit II	-
ECIAI39_RS01230	277439..279430	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
ECIAI39_RS01235	279420..280034	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
ECIAI39_RS01240	280034..280363	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
ECIAI39_RS01245	280375..281265	+	891	WP_000971336.1	heme o synthase	-
ECIAI39_RS01250	281450..281761	+	312	WP_001353405.1	DUF1778 domain-containing protein	Antitoxin
ECIAI39_RS01255	281745..282287	+	543	WP_000227787.1	GNAT family N-acetyltransferase	Toxin
ECIAI39_RS01260	282343..283278	-	936	WP_012311701.1	sel1 repeat family protein	-
ECIAI39_RS01265	283685..285049	+	1365	WP_001000978.1	MFS transporter	-
ECIAI39_RS01270	285177..285668	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
ECIAI39_RS01275	285836..286747	+	912	WP_000705836.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (19-96)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19946.24 Da
• Isoelectric Point: 8.8951

>T23415 WP_000227787.1 NC_011750:281745-282287 [Escherichia coli IAI39]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHRALPIKGVYLDADPAAINFYTRLGFVQLSA
RPNVFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T23415 NC_011750:281745-282287 [Escherichia coli IAI39]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCTGCTAAAGCTCTTATTGACAGACAAAGTGGTGAACTGATTGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAGATTGGTGTTGTCAGATTAGTCATGTTAGGGGTAGCGCG
GAAATATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCGTGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAACTTTTATACTCGCCTCGGCTTTGTTCAGCTTTCAGCG
AGGCCCAATGTTTTCGGTGCTGTACCGATGTTTTTAGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11370.09 Da
• Isoelectric Point: 10.4825

>AT23415 WP_001353405.1 NC_011750:281450-281761 [Escherichia coli IAI39]
MPGKTVALPDIDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT23415 NC_011750:281450-281761 [Escherichia coli IAI39]
ATGCCTGGAAAAACAGTCGCTCTACCCGATATCGATAAAACGCTGAAAAATGCGCGGGTTGAACTCAAAACCAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCAGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• AlphaFold DB: A0A829GE43

Antitoxin

• AlphaFold DB: A0A829G7G3