Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3264376..3265030 Replicon chromosome
Accession NC_011748
Organism Escherichia coli 55989

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag EC55989_RS16375 Protein ID WP_000244772.1
Coordinates 3264376..3264783 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag EC55989_RS16380 Protein ID WP_000354046.1
Coordinates 3264764..3265030 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EC55989_RS16355 3260333..3262066 - 1734 WP_000813220.1 single-stranded-DNA-specific exonuclease RecJ -
EC55989_RS16360 3262072..3262782 - 711 WP_000715208.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
EC55989_RS16365 3262807..3263703 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
EC55989_RS16370 3263815..3264336 + 522 WP_001055874.1 flavodoxin FldB -
EC55989_RS16375 3264376..3264783 - 408 WP_000244772.1 protein YgfX Toxin
EC55989_RS16380 3264764..3265030 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
EC55989_RS16385 3265273..3266253 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
EC55989_RS16390 3266506..3267486 + 981 WP_000399597.1 IS110-like element IS621 family transposase -
EC55989_RS16395 3267609..3268268 - 660 WP_000250274.1 hemolysin III family protein -
EC55989_RS16400 3268432..3268743 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 3266506..3267486 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T23405 WP_000244772.1 NC_011748:c3264783-3264376 [Escherichia coli 55989]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T23405 NC_011748:c3264783-3264376 [Escherichia coli 55989]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT23405 WP_000354046.1 NC_011748:c3265030-3264764 [Escherichia coli 55989]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT23405 NC_011748:c3265030-3264764 [Escherichia coli 55989]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References