Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 3127293..3127876 Replicon chromosome
Accession NC_011748
Organism Escherichia coli 55989

Toxin (Protein)


Gene name mazF Uniprot ID U9XFN8
Locus tag EC55989_RS15720 Protein ID WP_000254745.1
Coordinates 3127293..3127628 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag EC55989_RS15725 Protein ID WP_000581937.1
Coordinates 3127628..3127876 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EC55989_RS15705 3123180..3124478 - 1299 WP_000036723.1 phosphopyruvate hydratase -
EC55989_RS15710 3124566..3126203 - 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
EC55989_RS15715 3126431..3127222 - 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
EC55989_RS15720 3127293..3127628 - 336 WP_000254745.1 endoribonuclease MazF Toxin
EC55989_RS15725 3127628..3127876 - 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
EC55989_RS15730 3127954..3130188 - 2235 WP_000226815.1 GTP pyrophosphokinase -
EC55989_RS15735 3130236..3131537 - 1302 WP_000046785.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
EC55989_RS15740 3131594..3131896 + 303 Protein_2987 two-component sensor histidine kinase BarA -
EC55989_RS15745 3131952..3132649 + 698 Protein_2988 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12158.08 Da        Isoelectric Point: 8.4777

>T23404 WP_000254745.1 NC_011748:c3127628-3127293 [Escherichia coli 55989]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T23404 NC_011748:c3127628-3127293 [Escherichia coli 55989]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTATACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT23404 WP_000581937.1 NC_011748:c3127876-3127628 [Escherichia coli 55989]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT23404 NC_011748:c3127876-3127628 [Escherichia coli 55989]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYM3

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References