Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 2819947..2820572 Replicon chromosome
Accession NC_011748
Organism Escherichia coli 55989

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag EC55989_RS14200 Protein ID WP_000911329.1
Coordinates 2819947..2820345 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag EC55989_RS14205 Protein ID WP_000450524.1
Coordinates 2820345..2820572 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EC55989_RS14180 2815825..2816025 + 201 WP_000383836.1 YpfN family protein -
EC55989_RS14185 2816135..2816833 - 699 WP_000679823.1 esterase -
EC55989_RS14190 2816907..2818922 - 2016 WP_000829294.1 tRNA cytosine(34) acetyltransferase TmcA -
EC55989_RS14195 2818937..2819800 - 864 WP_001267498.1 neutral zinc metallopeptidase -
EC55989_RS14200 2819947..2820345 - 399 WP_000911329.1 tRNA(fMet)-specific endonuclease VapC Toxin
EC55989_RS14205 2820345..2820572 - 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
EC55989_RS14210 2820727..2821440 - 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
EC55989_RS14220 2821653..2822687 - 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
EC55989_RS14225 2822704..2823582 - 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
EC55989_RS14230 2823728..2824300 + 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
EC55989_RS14235 2824300..2824770 + 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T23403 WP_000911329.1 NC_011748:c2820345-2819947 [Escherichia coli 55989]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T23403 NC_011748:c2820345-2819947 [Escherichia coli 55989]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGATTGGAGCTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT23403 WP_000450524.1 NC_011748:c2820572-2820345 [Escherichia coli 55989]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT23403 NC_011748:c2820572-2820345 [Escherichia coli 55989]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAGGATGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References