Detailed information of TA system TA233957

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): stbDE/ParE-YafN
• Location: 191861..192383
• Replicon: plasmid pR886_1
• Accession: NZ_CP091602
• Organism: Klebsiella pneumoniae strain Nord77_R886

Toxin (Protein)

• Gene name: stbE
• Uniprot ID: A0A0C7KG00
• Locus tag: L5469_RS28620
• Protein ID: WP_038991638.1
• Coordinates: 192099..192383 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: stbD
• Uniprot ID: A0A2J4R0U8
• Locus tag: L5469_RS28615
• Protein ID: WP_004181777.1
• Coordinates: 191861..192109 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L5469_RS28595 (L5469_28600)	187070..187891	-	822	WP_004181772.1	hypothetical protein	-
L5469_RS28600 (L5469_28605)	187953..188306	-	354	WP_004181774.1	hypothetical protein	-
L5469_RS28605 (L5469_28610)	188451..189437	-	987	WP_040120328.1	hypothetical protein	-
L5469_RS28610 (L5469_28615)	189771..191570	-	1800	WP_043907032.1	ATP-dependent helicase	-
L5469_RS28615 (L5469_28620)	191861..192109	+	249	WP_004181777.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
L5469_RS28620 (L5469_28625)	192099..192383	+	285	WP_038991638.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
L5469_RS28625 (L5469_28630)	192400..192501	-	102	Protein_210	IS200/IS605 family transposase	-
L5469_RS28630 (L5469_28635)	192537..193763	+	1227	WP_040120327.1	RNA-guided endonuclease TnpB family protein	-
L5469_RS28635 (L5469_28640)	194034..194258	-	225	Protein_212	transposase	-
L5469_RS28640 (L5469_28645)	194337..194765	-	429	WP_077256229.1	IS200/IS605 family transposase	-
L5469_RS28645 (L5469_28650)	194801..195988	+	1188	WP_043907031.1	RNA-guided endonuclease TnpB family protein	-
L5469_RS28650 (L5469_28655)	196033..196404	-	372	WP_044816264.1	hypothetical protein	-
L5469_RS28655 (L5469_28660)	196401..196745	-	345	WP_014386518.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	dfrA14 / blaOXA-1 / aac(6')-Ib-cr / qnrB1 / blaNDM-1 / aph(3')-VI / mph(E) / msr(E) / armA / sul1 / qacE / aadA2 / dfrA12 / blaCTX-M-15 / aac(6')-Ib / blaTEM-1A / tet(D)	-	1..298468	298468

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-84)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10993.73 Da
• Isoelectric Point: 10.6516

>T233957 WP_038991638.1 NZ_CP091602:192099-192383 [Klebsiella pneumoniae]
MTYKLAFNESALKEWKKLGHTIREQFKKKLAERLLNPRVPASQLHGRKDQYKIKLRGAGYRLVYSVNDDVVTVTVIGVGK
RENDDIYHLTKHRN

Nucleotide

• Length: 285 bp

>T233957 NZ_LN890335:c865004-864627 [Achromobacter xylosoxidans]
ATGATCCTGGTTGACACTTCCATCTGGATAAACCATTTATCCGACGGCGAGCCATATCTGGTGCGCTTGCTGGAGGAAGA
AAGTGTATTGATGCACCCGTTCATCGCGGGTGAATTGGCGCTTGGTTCGTTGGGCCGGCGTGATGTCGTATTGGGTGCGA
TGTCGCTATTGCCTCAAGTCGTGCGTGCGAGTCATGACGAAGTCATGCACTTTCTTCATAGCGAGAGATTGTTTGGAAAG
GGCATTGGTTATGTGGATTTACACTTGTTGGCGTCCACGCGACTGACACCTGGCGCGGCGCTATGGACCAGGGACAAACG
GCTGCAGGCACTCGCCACCACCTTGGGCCTGGCGGTGGATCCCCCGAGATATCATTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8925.17 Da
• Isoelectric Point: 3.8209

>AT233957 WP_004181777.1 NZ_CP091602:191861-192109 [Klebsiella pneumoniae]
MAFQVLTTTAASITELKRDPMGTFNAGEGAPVAILNRNEPAFYCVPPALYAQLMELLEDQELGRIADERIGEAVIEVSLD
DL

Nucleotide

• Length: 249 bp

>AT233957 NZ_LN890335:c865200-864991 [Achromobacter xylosoxidans]
GTGCGTACGACTGTGACGCTCGATGACGACCTGCTCGAATTGGCCCGTACCTATACAGGCGTCCAGGAGACATCGGCGCT
CGTAACGCGAGCGTTGACCCAGCTCGTGCAGAAAGAGGCCGCGAAGCGGTTGGCCAAGCTTGGTGGTAGTTCGCCCAAGT
TGAAGCCTATCCCGCGGCGGCAGACGGAAACCTGCAATGATCCTGGTTGA

Structures

Toxin

• AlphaFold DB: A0A0C7KG00

Antitoxin

• AlphaFold DB: A0A2J4R0U8