Detailed information of TA system TA233955

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 37398..38308
• Replicon: plasmid pR886_1
• Accession: NZ_CP091602
• Organism: Klebsiella pneumoniae strain Nord77_R886

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A5Q9LR54
• Locus tag: L5469_RS27815
• Protein ID: WP_004181896.1
• Coordinates: 37838..38308 (+)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A2J4RLY9
• Locus tag: L5469_RS27810
• Protein ID: WP_004181895.1
• Coordinates: 37398..37841 (+)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L5469_RS27795 (L5469_27805)	34031..35518	+	1488	WP_004178082.1	group II intron reverse transcriptase/maturase	-
L5469_RS27800 (L5469_27810)	35604..36026	+	423	WP_004181893.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-
L5469_RS27805 (L5469_27815)	36026..37297	+	1272	WP_004181894.1	translesion error-prone DNA polymerase V subunit UmuC	-
L5469_RS27810 (L5469_27820)	37398..37841	+	444	WP_004181895.1	DUF2384 domain-containing protein	Antitoxin
L5469_RS27815 (L5469_27825)	37838..38308	+	471	WP_004181896.1	RES family NAD+ phosphorylase	Toxin
L5469_RS27820 (L5469_27830)	38418..38678	-	261	WP_004026352.1	hypothetical protein	-
L5469_RS27825 (L5469_27835)	39401..39559	+	159	WP_004181898.1	type I toxin-antitoxin system Hok family toxin	-
L5469_RS27830 (L5469_27840)	40162..41136	+	975	WP_043907013.1	hypothetical protein	-
L5469_RS27835 (L5469_27845)	41334..41711	-	378	WP_004181901.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	dfrA14 / blaOXA-1 / aac(6')-Ib-cr / qnrB1 / blaNDM-1 / aph(3')-VI / mph(E) / msr(E) / armA / sul1 / qacE / aadA2 / dfrA12 / blaCTX-M-15 / aac(6')-Ib / blaTEM-1A / tet(D)	-	1..298468	298468

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (30-89)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17549.81 Da
• Isoelectric Point: 4.6155

>T233955 WP_004181896.1 NZ_CP091602:37838-38308 [Klebsiella pneumoniae]
VIFYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T233955 NZ_LN885086:2924528-2924905 [Candidatus Nitrospira inopinata]
ATGGGGCGTCAGGAACGGCCGCTCGAATGGATTGGCAGTAGCTATAAAGATTTGATGGCGTTACCGGTTGCCGTTCGGCG
ATTCTTCGGGTACGCGTTGTCGCTGGCTCAGGCCGGCGATCAACATGATGATGCGAAGGTGCTCACGGGTTTCGGCAGCG
CCGGTGTGCTTGAAGTGGTCGAAGACGACAGGAGCGGCACCTATCGAGCGGTGTATACCGTGAAGTTCAAGGAGGCGGTC
TTCGTCCTGCATTGTTTCCAAAAAAAGAGTAAGCATGGTATCGCCACGCCGAAAGAGGATCTTGCCATCATCCGCGCCAG
GCTGAAAGTGGCTGAGGCGCTGGCCAAGGAGCGACGACATGAAAAAACGAGTCATTGA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16536.87 Da
• Isoelectric Point: 10.3013

>AT233955 WP_004181895.1 NZ_CP091602:37398-37841 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT233955 NZ_LN885086:2924886-2925221 [Candidatus Nitrospira inopinata]
ATGAAAAAACGAGTCATTGAAGGGATTGAGGTCTTTCGCGGGTCTGGCAATGTCTTCGCCGACTTGGATCTGCCAGATGC
CGATACGCTCCAGATCAAGACCGGGCTGGTGATCGAGATCCGGAGAGCCATTCGCCAACAAGGGCTGACCCAGCAAGCCG
CGGCCACACGGATGGGGCTCACCCAACCGAAGGTCTCCAGCATGATGCGAGGGGACTTCTCCAATGTGTCCGAACGCAAA
CTGATGGAGTGCCTGAATCGGCTCGGCTACGATATTGAAATCAAAGTTCGGCCAGCCTCCGCTCGCATCGGACATCTGAT
GCTGGCGCTACCGTGA

Structures

Toxin

• AlphaFold DB: A0A5Q9LR54

Antitoxin

• AlphaFold DB: A0A2J4RLY9