Detailed information of TA system TA233954

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 5011749..5012406
• Replicon: chromosome
• Accession: NZ_CP091601
• Organism: Klebsiella pneumoniae strain Nord77_R886

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: -
• Locus tag: L5469_RS25835
• Protein ID: WP_004181233.1
• Coordinates: 5011749..5012159 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: W8UQ37
• Locus tag: L5469_RS25840
• Protein ID: WP_002916312.1
• Coordinates: 5012140..5012406 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L5469_RS25815 (5007749)	5007749..5009482	-	1734	WP_004149758.1	single-stranded-DNA-specific exonuclease RecJ	-
L5469_RS25820 (5009488)	5009488..5010201	-	714	WP_002916301.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
L5469_RS25825 (5010224)	5010224..5011120	-	897	WP_004144729.1	site-specific tyrosine recombinase XerD	-
L5469_RS25830 (5011221)	5011221..5011742	+	522	WP_004144730.1	flavodoxin FldB	-
L5469_RS25835 (5011749)	5011749..5012159	-	411	WP_004181233.1	protein YgfX	Toxin
L5469_RS25840 (5012140)	5012140..5012406	-	267	WP_002916312.1	FAD assembly factor SdhE	Antitoxin
L5469_RS25845 (5012652)	5012652..5013635	+	984	WP_002916313.1	tRNA-modifying protein YgfZ	-
L5469_RS25850 (5013786)	5013786..5014445	-	660	WP_002916317.1	hemolysin III family protein	-
L5469_RS25855 (5014609)	5014609..5014920	-	312	WP_004144734.1	N(4)-acetylcytidine aminohydrolase	-
L5469_RS25860 (5014970)	5014970..5015698	+	729	WP_002916321.1	MurR/RpiR family transcriptional regulator	-
L5469_RS25865 (5015817)	5015817..5017250	+	1434	WP_004181234.1	6-phospho-beta-glucosidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-132)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 16121.92 Da
• Isoelectric Point: 11.1565

>T233954 WP_004181233.1 NZ_CP091601:c5012159-5011749 [Klebsiella pneumoniae]
VVLWQSDLRISWRAQWFSLLLHGVVAALVLLVPWPLSYTPIWLLLLSLVVFDCVRSQRRIHARRGEIKLLTDSRLRWQNA
EWEILGTPWVINSGMLLRLRHVDTRRGQHLWLAADSMDAEEWRDLRRLVLQKPAQE

Nucleotide

• Length: 411 bp

>T233954 NZ_LN885086:1740113-1740403 [Candidatus Nitrospira inopinata]
GTGATTGAATCTTTTGCCAATCGACTTGCGGAAGATCTTTTCGACGATCGGCAATCAAAGGCCGTTCTGAAATTTCCGTC
CGATCTGATCCGTACGGCCCGTCGCAAGCTGCTCTATTTGCACGATGCGTCGGAAATCGGGGATCTGCGCGAGCCACCGG
GAAATCGATTGGAAGCGCTGAAGGGCGAGTGGAAGGGGTTTTACTCCATTCGGATCAATGATCAATGGCGGGTTGTATTT
CGTTGGCATGACGGTAATGCGTACGAGGTTCAAATCATCGACTATCATTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10480.98 Da
• Isoelectric Point: 5.1116

>AT233954 WP_002916312.1 NZ_CP091601:c5012406-5012140 [Klebsiella pneumoniae]
MDINNKARIHWACRRGMRELDISIMPFFEYEYDTLSDADKQLFIRLLENDDPDLFNWLMNHGKPADAELQRMVTLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT233954 NZ_LN885086:1740440-1740706 [Candidatus Nitrospira inopinata]
ATGAGACTCCCCAAGAACCCATTTCATCCGGGTGAGATGCTGCTCGAAGAGTTTCTCATCCCCAGGAAGATGACTCAGGC
GGCGTTGGCGAAAAAGCTTGGCTGGACGAGAGCTCGCCTGAACGAGCTGATTAAGGGCAAGCGCGACATTACGGCGGACT
CCGCCCTGGATTTGGCACGTGTGCTTGGCACGTCCGCCAAACTTTGGATGAATCTCCAGGCGACATTTGATCTCGACAAG
GCGATGCGAAAAAGAAAGATCGCCTGA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GY41