Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 569013..569638 Replicon chromosome
Accession NZ_CP091601
Organism Klebsiella pneumoniae strain Nord77_R886

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag L5469_RS04265 Protein ID WP_002882817.1
Coordinates 569255..569638 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag L5469_RS04260 Protein ID WP_004150355.1
Coordinates 569013..569255 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L5469_RS04235 (565004) 565004..565603 + 600 WP_004151865.1 glucose-1-phosphatase -
L5469_RS04240 (565597) 565597..566457 + 861 WP_004146232.1 virulence factor BrkB family protein -
L5469_RS04245 (566454) 566454..566891 + 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
L5469_RS04250 (566936) 566936..567877 + 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
L5469_RS04255 (567891) 567891..568808 - 918 WP_004181612.1 alpha/beta hydrolase -
L5469_RS04260 (569013) 569013..569255 + 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
L5469_RS04265 (569255) 569255..569638 + 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
L5469_RS04270 (569812) 569812..570741 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
L5469_RS04275 (570738) 570738..571373 - 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
L5469_RS04280 (571370) 571370..572272 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T233940 WP_002882817.1 NZ_CP091601:569255-569638 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T233940 NZ_LN871187:c5254910-5254632 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT233940 WP_004150355.1 NZ_CP091601:569013-569255 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT233940 NZ_LN871187:c5254620-5254315 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTTGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References