Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 53879..54522 Replicon plasmid pR886_3
Accession NZ_CP091600
Organism Klebsiella pneumoniae strain Nord77_R886

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag L5469_RS01440 Protein ID WP_001044770.1
Coordinates 54106..54522 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag L5469_RS01435 Protein ID WP_001261282.1
Coordinates 53879..54109 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L5469_RS01400 (L5469_01400) 49451..50317 - 867 WP_004118283.1 replication initiation protein -
L5469_RS01405 (L5469_01405) 50852..50956 + 105 WP_032409716.1 hypothetical protein -
L5469_RS01410 (L5469_01410) 51085..51342 + 258 WP_000764642.1 hypothetical protein -
L5469_RS01415 (L5469_01415) 51400..52176 - 777 WP_000015958.1 site-specific integrase -
L5469_RS01420 (L5469_01420) 52173..52916 - 744 WP_000129823.1 hypothetical protein -
L5469_RS01425 (L5469_01425) 52967..53317 - 351 WP_000493378.1 hypothetical protein -
L5469_RS01430 (L5469_01430) 53461..53922 - 462 WP_072202616.1 hypothetical protein -
L5469_RS01435 (L5469_01435) 53879..54109 + 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
L5469_RS01440 (L5469_01440) 54106..54522 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
L5469_RS01445 (L5469_01445) 54596..55285 + 690 Protein_68 AAA family ATPase -
L5469_RS01450 (L5469_01450) 55340..56037 + 698 WP_223174630.1 IS1 family transposase -
L5469_RS01455 (L5469_01455) 56122..56982 - 861 WP_000027057.1 broad-spectrum class A beta-lactamase TEM-1 -
L5469_RS01460 (L5469_01460) 57165..57482 - 318 Protein_71 recombinase family protein -
L5469_RS01465 (L5469_01465) 57682..58506 - 825 WP_000722315.1 oxacillin-hydrolyzing class D beta-lactamase OXA-9 -
L5469_RS01470 (L5469_01470) 58566..59354 - 789 WP_001206315.1 ANT(3'')-Ia family aminoglycoside nucleotidyltransferase AadA1 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid blaSHV-187 / ARR-3 / ere(A) / ant(3'')-Ia / cmlA1 / qacE / sul1 / aph(6)-Id / aph(3'')-Ib / sul2 / blaTEM-1A / blaOXA-9 / aac(6')-Ib / blaCTX-M-15 / aac(3)-IId - 1..69630 69630


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T233938 WP_001044770.1 NZ_CP091600:54106-54522 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T233938 NZ_LN871187:c4399210-4398455 [Pseudomonas aeruginosa]
GTGAGCGAGATCTGGCGACAGTGCAAGGGTGAGCGGCATATCCGACCGCTCCAGGGGCGCCTGGTGCGGTTGGTGGAGAG
CCAGGAGCAGGTGGCGACCCTGCAATTGGTCGACACCCTGGAAGAACAGGCGTTGCTCGAGGAACTGCTCGAGTCGAGCA
AGCCGCCGGTGCCGGCGGATGCCGAGCCATTGCATTACCTGTTGAAGACCCCGTTCCGTTATCCGCCCTTGCGCTGGGGG
TCGCGGTTTGGCCGTCGCCACGAGCCAAGCCTGTTCTACGCCGCGCTGAAGCTGGAAACGGCCATGGCCGAAAGCGCGTA
TTACCGTTGCGTCCTGTGGAGTGGCATGGTGGTGCCGCCACCGAGCGGGAGGATTCTGTCCGAGCACGCTTCGTTCGAGG
CGGGCTGGAAGGTGGAGCGGGGAATCCGTCTGCAGGCGCCGCCTTTCTCCGATCACGAGGCTGCGCTGACCGACATCGCC
GACTACCGCGCTCCCCAGGAACTGGGAAGTGCCATGCGCAGCGCCGGGGTGCAGGCATTCGAATACCGTTCGGCGCGTTG
CCCGGAGCGCGGCTGCAATGTCGCCCTGTTCACGCCCGCGGCCTTCACCGAAAAGCGTCCTCGCAACCTGACGCCCTGGC
TGTGCGAAACCACGGCCGGTTACGTGGCCTTCAAGCCGGCGCATGTGCCGGGGTCGCCGAAAATCTTCTCCTGGGAGCTG
TTCCTGGTGGACGGAAAGTTGCCGCATCCGGCGTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT233938 WP_001261282.1 NZ_CP091600:53879-54109 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT233938 NZ_LN871187:c4399713-4399345 [Pseudomonas aeruginosa]
ATGAGCGCTCTCATCAAGGAACGTCCCAGCGCCGATGCCGTCCTGGCCAAGGCCGTCCTGGCCGCGCGCGAGCAATTGGG
GCTGACGCAGCTCGAACTGGCCGGCATCGTCGGCGTCGATCGCAGCGCCATCAGCCGCTGGAAGACCCAGGGCTTGCGGG
TGGACAGCAAGACCGGCGAGCTGGCTCTGCTGCTGGTGCGAGTCTATCGCGCACTGTATGCCCTGTTCGGCGGGCAGCAG
GAGGACATGCGCCACTTCCTGCGCACTCCCAACCATCACCTGGCGGGCGAGCCGCTGGCACTGATGGGACAGGTGCAGGG
CCTGGTTCATGTGCTGGAATACCTCGATGCGATCCGCGGCAAGGTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References