Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 189418..190154 Replicon plasmid pR886_2
Accession NZ_CP091599
Organism Klebsiella pneumoniae strain Nord77_R886

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag L5469_RS00985 Protein ID WP_003026803.1
Coordinates 189672..190154 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag L5469_RS00980 Protein ID WP_003026799.1
Coordinates 189418..189684 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L5469_RS00935 (L5469_00935) 185480..185842 - 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -
L5469_RS00940 (L5469_00940) 185892..186242 - 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
L5469_RS00945 (L5469_00945) 186600..186869 + 270 WP_004152102.1 hypothetical protein -
L5469_RS00950 (L5469_00950) 186857..187432 + 576 WP_004152103.1 hypothetical protein -
L5469_RS00955 (L5469_00955) 187463..187957 + 495 WP_004152104.1 DNA-binding protein -
L5469_RS00960 (L5469_00960) 188001..188369 + 369 WP_004152105.1 hypothetical protein -
L5469_RS00965 (L5469_00965) 188403..188606 + 204 WP_004152106.1 HHA domain-containing protein -
L5469_RS00970 (L5469_00970) 188655..188912 + 258 WP_004152107.1 hypothetical protein -
L5469_RS00975 (L5469_00975) 188988..189242 + 255 WP_004152108.1 hypothetical protein -
L5469_RS00980 (L5469_00980) 189418..189684 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
L5469_RS00985 (L5469_00985) 189672..190154 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
L5469_RS00990 (L5469_00990) 190366..191712 + 1347 WP_020314316.1 ISNCY family transposase -
L5469_RS29205 191873..192004 + 132 WP_004218042.1 hypothetical protein -
L5469_RS00995 (L5469_00995) 192213..193378 - 1166 Protein_199 IS3 family transposase -
L5469_RS01000 (L5469_01000) 193555..194517 - 963 WP_004152113.1 zinc metalloprotease HtpX -
L5469_RS01005 (L5469_01005) 194504..194992 - 489 WP_004152114.1 phosphate-starvation-inducible PsiE family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid catA1 - 1..211798 211798


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T233937 WP_003026803.1 NZ_CP091599:189672-190154 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T233937 NZ_LN871187:3466694-3467272 [Pseudomonas aeruginosa]
ATGTCCCTCGAACTGCTGAGCAACCGCCTCGAATTCCTCCGCGAAGCCGAACGCCTGAAAGACGTCCTGCGCTCCGCGCA
CACCTCCTCCGGCCGCCCGGAAAGCACCGCCGAACATAGCTGGCGGCTGTGCCTGATGGCACTGGCCTTCGAGGACCAAC
TGGCCGGCCTGGACCTCGGCAAGGTCCTGCGCATGTGCGTGGTCCACGACCTCGGCGAAGCGATCCACGGCGACATCCCC
GCCGTCGAGCAGGCCGCGCACCCGGACAAAGGCGAACAGGAACGCGCCGACCTGTTGCAGCTGACCCGTCACCTCGACGC
TCCGCTGCGCGACCGCCTGCTCGCCCTGTGGGACGAATACGAGCGCGGCGAGTCCGCCGAAGCCCTGGCAGTGAAGGCCC
TGGACAAGCTGGAAACCCTGCTCCAGCACACCCAGGGCGACAATCCGGCGGACTTCGACTATGCCTTCAACCTCGACTAC
GGCCGTCGCTATACCGACCGCGCACCGCTGTTCAAGACCCTGCGCGAGCTACTCGACGCCAGGACCCGACAGCGCCTCGC
AGTTCGGCGCGACGCCTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT233937 WP_003026799.1 NZ_CP091599:189418-189684 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT233937 NZ_LN871187:3466051-3466605 [Pseudomonas aeruginosa]
ATGACCGAGCCTCTCCCTTCTTCGTCCCTGGGCCCCGCCCTGCGTCGCTGGCGCCTGCTGCACCGGGTCAAGCAAACGCA
TGCCGCCGAATTGCTCGGCGTGGCGCAATCGACCATCTCGCGCTGGGAGTCCGGTACCCAGGCGCTGCTGGTGGAGGAAC
GGGCACGCCTGGAGCGTCTGCTCGGCGCCCGCCTGGAAGCCGCCGCCGACCAGGCACTGGCCCGCCTGGTCGAGGCCAAC
CCGCAACCCGTGCACCTGATCTGCGACCTCACCCATCGCCTGTTGGCCTGTTCGCCGGCACGCGCCGCGCAATTCGGCGT
GCCTCTCGGCGAACTGCTCGACCGTTCGCTGTGGCCCTATTGCAGCGAGGAAATCCTCCGCCAGGAAGCCACCCTCGAGG
AACTCGGCTGGCGCGAACTGCTGGCACCGCCGGCGCTGGAGTTCGCCAGCGGAGCGAACGCCTCGGCCATCGTGCCGATC
CGGCGCAGCCGTTGCCGCTGGACCCGCATGACCCTCTCGGACGGACGCGCGGTGCGCCTGGTGGAAACCCTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References