Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 2541901..2542637 Replicon chromosome
Accession NZ_CP091594
Organism Klebsiella pneumoniae strain Nord5-1_R48

Toxin (Protein)


Gene name tacT Uniprot ID A0A4S4Y1H2
Locus tag L5467_RS15450 Protein ID WP_032433360.1
Coordinates 2542155..2542637 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag L5467_RS15445 Protein ID WP_003026799.1
Coordinates 2541901..2542167 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L5467_RS15420 (2537547) 2537547..2538686 + 1140 WP_032433368.1 mannitol dehydrogenase -
L5467_RS15425 (2538715) 2538715..2539377 + 663 WP_032433366.1 dihydroxyacetone kinase subunit DhaL -
L5467_RS15430 (2539361) 2539361..2540365 + 1005 WP_032433364.1 dihydroxyacetone kinase subunit DhaK -
L5467_RS15435 (2540383) 2540383..2541015 + 633 WP_032433362.1 3-hexulose-6-phosphate synthase -
L5467_RS15440 (2541025) 2541025..2541588 + 564 WP_032433361.1 6-phospho-3-hexuloisomerase -
L5467_RS15445 (2541901) 2541901..2542167 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
L5467_RS15450 (2542155) 2542155..2542637 + 483 WP_032433360.1 GNAT family N-acetyltransferase Toxin
L5467_RS15455 (2542998) 2542998..2543597 - 600 WP_032432064.1 helix-turn-helix transcriptional regulator -
L5467_RS15460 (2543810) 2543810..2544754 - 945 WP_077254249.1 fimbrial protein -
L5467_RS15465 (2544766) 2544766..2545344 - 579 WP_032432061.1 type 1 fimbrial protein -
L5467_RS15470 (2545348) 2545348..2546088 - 741 WP_050597964.1 molecular chaperone -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2509846..2553580 43734


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(72-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17421.02 Da        Isoelectric Point: 9.6047

>T233924 WP_032433360.1 NZ_CP091594:2542155-2542637 [Klebsiella pneumoniae]
VGRVTAPEPLSSSHQLAEFVSGETVLDEWLKHRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTETTGRLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYTHHGFTASQTHERTLFLKLP

Download         Length: 483 bp

>T233924 NZ_LN868939:1767959-1768240 [Nocardia farcinica]
GTGATCAGATCGTTCGGCGACAAGGAGACCGAACGGCTGTGGCGTCGTGAGCGGGTGCGGTCACTCGATCCGAGGATCCA
TAGGGTTGCACTTCGCAAACTTCGTCAGGTGGGATCCGCTGGGTCTCTTGAGGACCTTCGCATCCCGCCTGGCAACCGAC
TCGAAGCACTCAAGGGTGATCGGGTGGGGCAGCACAGCATTAGGATCAACGACCAGTGGCGGATCTGTTTCCGATGGACG
GACGCCGGACCGGAGGAGGTGGAGATCGTTGACTACCACTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT233924 WP_003026799.1 NZ_CP091594:2541901-2542167 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT233924 NZ_LN868939:1768227-1768529 [Nocardia farcinica]
TTGACTACCACTGACAAGATCGCGCCGATTCACCCCGGAGAAGTCCTCATGGAGGATTTCATCGAGGGCTTCGGGATCAC
GCAGAACAAACTCGCCGTGTCGATCGGAGTGCCACCTCGGCGGATCAACGAGATCGTGCACGGCAAGCGGGGGGTCACCG
CAGACACCGCGCTGCGGCTTGCGAAGTACTTCGGTACCTCGGCGGAGTTCTGGCTCAACCTGCAGAGTCACTACGAGCTG
GATCGTGCTGAGGACATCGCTGGGGAACAGATTGCTGCAATCGTTCCACTGGGTGCTGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4S4Y1H2

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References