Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 89077..89720 Replicon plasmid pR48_4
Accession NZ_CP091593
Organism Klebsiella pneumoniae strain Nord5-1_R48

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag L5467_RS03140 Protein ID WP_001044770.1
Coordinates 89304..89720 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag L5467_RS03135 Protein ID WP_001261282.1
Coordinates 89077..89307 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L5467_RS03095 (L5467_03100) 84275..84748 + 474 WP_004152341.1 YkgJ family cysteine cluster protein -
L5467_RS03100 (L5467_03105) 84840..85070 + 231 WP_011977773.1 hypothetical protein -
L5467_RS03105 (L5467_03110) 85962..86744 - 783 WP_004152340.1 site-specific integrase -
L5467_RS03110 (L5467_03115) 86744..87076 - 333 WP_004152339.1 hypothetical protein -
L5467_RS03115 (L5467_03120) 87083..87481 - 399 WP_004171440.1 hypothetical protein -
L5467_RS03120 (L5467_03125) 87507..87836 - 330 WP_004152337.1 hypothetical protein -
L5467_RS03125 (L5467_03130) 87864..88172 - 309 WP_004152336.1 hypothetical protein -
L5467_RS03130 (L5467_03135) 88659..89120 - 462 WP_072093212.1 hypothetical protein -
L5467_RS03135 (L5467_03140) 89077..89307 + 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
L5467_RS03140 (L5467_03145) 89304..89720 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
L5467_RS03145 (L5467_03150) 89794..90483 + 690 Protein_108 AAA family ATPase -
L5467_RS03150 (L5467_03155) 90538..91235 + 698 WP_223174630.1 IS1 family transposase -
L5467_RS03155 (L5467_03160) 91253..91558 + 306 Protein_110 SDR family oxidoreductase -
L5467_RS03160 (L5467_03165) 91650..92294 - 645 WP_014386481.1 quinolone resistance pentapeptide repeat protein QnrB1 -
L5467_RS03165 (L5467_03170) 92389..92790 - 402 Protein_112 helix-turn-helix domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaSHV-12 / qnrB1 - 1..96023 96023
- flank IS/Tn - - 82887..84155 1268


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T233916 WP_001044770.1 NZ_CP091593:89304-89720 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T233916 NZ_LN854573:c5766167-5765283 [Pseudomonas sp. URMO17WK12:I11]
ATGTCGTACGCCATCGATAATCTCGTTTCGCCGCTCACGGCTGACCTCCAGGCACAACTGGCCCTGAACTTTCCGGTGCG
TACGCTGGAGATCGCCGGCGGTCGCCAAGCCTTTCGCGAATGCGGGGCCGGGCCGGCCATTGTGTTGTTGCACGGGATCG
GCTCAGGCTCGGCGTCGTGGTTAGAGGTGGCGCAGGAGCTTGGCCAGAGCGCCCGGGTGATTGCCTGGGACGCGCCCGGC
TACGGTGATTCCACGCCGCTGAGATCGCTGGCCCCCGACGCTTCGGACTACGCCGAACGACTGCTGCAACTGCTCGACGC
ACTGGGCATCCAGCGCTGTGTGCTGGTGGGTCATTCCCTGGGCGCGATTACTGCCACGGCGTTCGCCAGCGGCGTGCAGC
AGCAACGGATCAGCCGGCTGGTCTTGATCAGCCCGGCCCAAGGCTATGGCCATCCATCACGCGCGTTGCAGCGTCAGGAA
GTGCGCAACAAACGCCTGCGTGCCCTGGAACAACTGAGCATTCAGCAGATGGCCGAGCAACGCAGTCCCTACATGCTTTC
ACCCGCCGCCAGCCCGAAAGCACTGGCCTGGGTGCGATGGAACATGGCGCGGCTCAATCCCGATGGCTATCGCCAGGCCA
TTGAACTGCTGTGCAGCGATGACCTGCTGCGGCACCCACCGCTGTCGATGCCTTGCGAAGTGCATTGCGGCGAGGCCGAC
GGCATCACCGCGCCCGAAAGCTGCCTGACCCTGGCCAAGGCGTTGGGCGCCACGTTCAACCTGATTGCCGATGCCGGGCA
TGCCAGCCCTATTGAACAACCTTATGTCGTGGCGGGCCGTCTGGCCTTTGCCATTAAAGAATCCCTGACAGGTGCATCTC
TATGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT233916 WP_001261282.1 NZ_CP091593:89077-89307 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT233916 NZ_LN854573:c5765286-5764453 [Pseudomonas sp. URMO17WK12:I11]
ATGAACGATTCAGATCTGCTGAAGGACGCTCAGGACAAATACATCGTGCCGGGCCTGGAGCGCGGTCTGCTGCTGCTCTG
CGAATTCAGCCGGCGCAATCGCACGCTGACAGCGCCAGAACTGGCCCGGCGTCTGGCACTGCCACGCTCGACCATATTTC
GTCTGCTGTCGACCCTGGAAACCATGGGGTTTGTCACCCGCAGCGGCAATGAATACCGCTTGGGCATGTCGGTGCTACGC
CTGGGCTTTGAATACCTGGCTTCGCTGGAACTGACCGAACTGGGCCAGCCGCTGCTGGCTCGCTTGTGCGACCGCCTCAA
CTACCCGAGCAACCTGGTGGTGCGCGATGGTCGTTCGATCGTTTACGTGGCCAAGGTCTCGCCGCCGTCAGTGTTCTCCA
GTGCGGTCAACGTCGGCACTCGCCTGCCCGCCCACGCCACTGTCCTCGGACGAATTCTGCTCGAAGACTTGTCGCTGGCC
GAGTTGCGTGAGCTGTACCCGGAAGACCATCTGGAGCAGTTCTCGCCGTGCACGCCGAAAACCGTGATGGAACTGTTCGA
CCTGGTTCAGGCTGACCGCCAGCGCGGTTTCGTCAGCAGCGAAGGCTTCTTCGAGTCAACGATCTCGACCGTCGCCGCCC
CGGTGCGCGATGAGACGAGCAGGATCGTCGCCGCGCTGGGCGTCACGATCCCGACCGCACAGATCGCTCACGTCAACTTC
AACGAGTTGCTCCTGCAAGTGCGCCGCAGCGCCGACGAACTGTCGCGCCTGCTCAACTACAACAGCGCTGCTGCCCATGA
CGGGCACCGCGTCACTGCATTGATGAGGGATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References