Detailed information of TA system TA233914

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 47260..48170
• Replicon: plasmid pR48_1
• Accession: NZ_CP091588
• Organism: Klebsiella pneumoniae strain Nord5-1_R48

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: L5467_RS00225
• Protein ID: WP_004883378.1
• Coordinates: 47260..47730 (-)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A6P1V3Q9
• Locus tag: L5467_RS00230
• Protein ID: WP_004026357.1
• Coordinates: 47727..48170 (-)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L5467_RS00205 (L5467_00205)	43463..43621	-	159	WP_004181898.1	type I toxin-antitoxin system Hok family toxin	-
L5467_RS00210 (L5467_00210)	43693..44826	+	1134	WP_171888422.1	IS481-like element ISKpn28 family transposase	-
L5467_RS00220 (L5467_00220)	46891..47151	+	261	WP_004026352.1	hypothetical protein	-
L5467_RS00225 (L5467_00225)	47260..47730	-	471	WP_004883378.1	RES family NAD+ phosphorylase	Toxin
L5467_RS00230 (L5467_00230)	47727..48170	-	444	WP_004026357.1	DUF2384 domain-containing protein	Antitoxin
L5467_RS00235 (L5467_00235)	48270..49541	-	1272	WP_004181894.1	translesion error-prone DNA polymerase V subunit UmuC	-
L5467_RS00240 (L5467_00240)	49541..49963	-	423	WP_004181893.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-
L5467_RS00245 (L5467_00245)	50318..50887	-	570	Protein_49	transposase	-
L5467_RS00250 (L5467_00250)	50889..51104	+	216	Protein_50	IS200/IS605 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..196916	196916
	-	inside	IScluster/Tn	-	-	43693..50671	6978

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (30-89)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17500.82 Da
• Isoelectric Point: 4.6155

>T233914 WP_004883378.1 NZ_CP091588:c47730-47260 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVLKAQQIPFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T233914 NZ_LN854573:c2488862-2488500 [Pseudomonas sp. URMO17WK12:I11]
ATGGTGCGTGGGCAGGCCCCGCAGCGGGGCGACGTCTATTGGGTTGACCCCAATCCGGTTGCTGGACGAGAGATGATGAG
TCGGCATCGCTTCGTGGTGATAACGCCAAGGGAAATCAATGCTCTGGGTGTCAGCATGACGGTCCCTATCACCAGCGGCG
GCAGCTTCAGTCGAAACTCGGGCCTTGCGGTTGTCGTGTCAGGACATGACACGACTGGGGTCGCGGTGTGTAATCAGGTG
CGCAGTTTTGATATTGAACAGCGCGTCCGTGATGGCTCTGCCAAATTTATCGAGCGTCTTGATGACGTGACAATGGCCGA
CATCGTTGCTCGAGTCGTCAGTTCCATCGATCCACTGGATTAG

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16480.80 Da
• Isoelectric Point: 10.2498

>AT233914 WP_004026357.1 NZ_CP091588:c48170-47727 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT233914 NZ_LN854573:c2489131-2488862 [Pseudomonas sp. URMO17WK12:I11]
ATGTCCGTGACTTCCAAAATCCGCCGTCAAGGTGGAGCCGCCGTGATAACTCTCCCACCTACCTTACTGAAACTCCTGCA
TCTGGAGGTAGGGGCACAGTTGGAGCTCAACGTGGTTAATGGTGAGTTGATTGCTCGCCCCGTTGTGCCGTCTTCTCGTA
AACGCTACAGCTTGACTGAGTTGCTTGAAGGCGCTGAAGAAATGGCTGAATTGAATGCGCTGACATCGTCTGCCCGCGAA
GGTGATCCAGTTGGCCGGGAGTTGGGTTGA

Structures

Antitoxin

• AlphaFold DB: A0A6P1V3Q9