Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 97274..98010 Replicon plasmid pR85_2-1
Accession NZ_CP091584
Organism Klebsiella pneumoniae strain Nord9_R85

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag L5468_RS28060 Protein ID WP_003026803.1
Coordinates 97274..97756 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag L5468_RS28065 Protein ID WP_003026799.1
Coordinates 97744..98010 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L5468_RS29665 93141..93272 - 132 WP_004218042.1 hypothetical protein -
L5468_RS28045 (L5468_28050) 93433..94779 - 1347 WP_020314316.1 ISNCY family transposase -
L5468_RS28050 (L5468_28055) 95009..95641 + 633 WP_001567369.1 hypothetical protein -
L5468_RS28055 (L5468_28060) 95670..97073 - 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
L5468_RS28060 (L5468_28065) 97274..97756 - 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
L5468_RS28065 (L5468_28070) 97744..98010 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
L5468_RS28070 (L5468_28075) 98186..98440 - 255 WP_004152108.1 hypothetical protein -
L5468_RS28075 (L5468_28080) 98516..98773 - 258 WP_004152107.1 hypothetical protein -
L5468_RS28080 (L5468_28085) 98822..99025 - 204 WP_004152106.1 HHA domain-containing protein -
L5468_RS28085 (L5468_28090) 99059..99427 - 369 WP_004152105.1 hypothetical protein -
L5468_RS28090 (L5468_28095) 99471..99965 - 495 WP_004152104.1 DNA-binding protein -
L5468_RS28095 (L5468_28100) 99996..100571 - 576 WP_004152103.1 hypothetical protein -
L5468_RS28100 (L5468_28105) 100559..100828 - 270 WP_004152102.1 hypothetical protein -
L5468_RS28105 (L5468_28110) 101186..101536 + 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
L5468_RS28110 (L5468_28115) 101586..101948 + 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid tet(D) - 1..214248 214248


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T233912 WP_003026803.1 NZ_CP091584:c97756-97274 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T233912 NZ_LN854557:2654931-2655314 [Sodalis glossinidius str. 'morsitans']
ATGAAATGGATCAGCGCCCAGGAGGTGATAGCGTTTCATGATCGCCTACTTGCAGCTTTGCCGGGCGTGCAGAGGATGCC
AGAACCGGGAAGAGCAGAGGCGATCATTTATCGTGTTCAGAACCAGCTGTACTATGAAGGCACTGAGGATATTCACGAAC
TCTCTGCGATGTATCTTGTCGCTATTTCGCGTGGCCATATCTTCAATGACGGCAATAAGCGAACCGCCTTTTTCGTTGCG
ATGGCTTTTTTAAAGCGCAATGGCATTGATGTCCGTGATGAAGGTGACGAACTGGAAGAACTGACGGTATCCGCAGCGAT
GGGTTCAGTAAGCAGCGGTGATGTTGCTGCTGTTTTGCGCCGACTATCCATCAATCCCCACTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT233912 WP_003026799.1 NZ_CP091584:c98010-97744 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT233912 NZ_LN854557:2654713-2654934 [Sodalis glossinidius str. 'morsitans']
ATGCGGACCTATACCACATCTCAGGCCCGGCAAAATATTGCAGAGGTAATGGAAGCGGCTACGGCTGGTGAGCCTGTAGA
AATAACCCGCCGCGATGGCTCTGCCGCCGTACTTATCAGCCGCGATGATTTCAACACGTGGCAGGAAACAAAGCTGGATG
CTGAATTTGCCGAAATTATGGGGCGGCACGGTTGCACTATTAAGGCGCTGGCCGACAGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References