Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 187854..188764 Replicon plasmid pR85_1
Accession NZ_CP091583
Organism Klebsiella pneumoniae strain Nord9_R85

Toxin (Protein)


Gene name - Uniprot ID A0A663AYG0
Locus tag L5468_RS27240 Protein ID WP_004026354.1
Coordinates 188294..188764 (+) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A6P1V3Q9
Locus tag L5468_RS27235 Protein ID WP_004026357.1
Coordinates 187854..188297 (+) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L5468_RS27210 (L5468_27215) 183011..183226 - 216 Protein_197 IS200/IS605 family transposase -
L5468_RS27215 (L5468_27220) 183228..183797 + 570 Protein_198 transposase -
L5468_RS27220 (L5468_27225) 184165..185652 - 1488 WP_004178082.1 group II intron reverse transcriptase/maturase -
L5468_RS27225 (L5468_27230) 186061..186483 + 423 WP_004181893.1 translesion error-prone DNA polymerase V autoproteolytic subunit -
L5468_RS27230 (L5468_27235) 186483..187754 + 1272 WP_004181894.1 translesion error-prone DNA polymerase V subunit UmuC -
L5468_RS27235 (L5468_27240) 187854..188297 + 444 WP_004026357.1 DUF2384 domain-containing protein Antitoxin
L5468_RS27240 (L5468_27245) 188294..188764 + 471 WP_004026354.1 RES family NAD+ phosphorylase Toxin
L5468_RS27250 (L5468_27255) 189613..190017 - 405 Protein_205 aminoglycoside adenylyltransferase family protein -
L5468_RS27255 (L5468_27260) 190110..191369 - 1260 WP_025368619.1 chloramphenicol efflux MFS transporter CmlA4 -
L5468_RS27260 (L5468_27265) 191668..192141 - 474 WP_001830196.1 trimethoprim-resistant dihydrofolate reductase DfrA15 -
L5468_RS27265 (L5468_27270) 192298..193311 + 1014 WP_000845048.1 class 1 integron integrase IntI1 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid oqxB / oqxA / blaTEM-1B / tet(B) / cmlA1 / dfrA15 / sul2 / catA2 / aac(6')-Iq / ant(3'')-Ia / qacE / sul1 / blaCTX-M-2 - 1..258013 258013
- flank IS/Tn - - 183288..183797 509


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17515.79 Da        Isoelectric Point: 4.6155

>T233911 WP_004026354.1 NZ_CP091583:188294-188764 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T233911 NZ_LN854557:2137267-2137347 [Sodalis glossinidius str. 'morsitans']
AAGCCTACAAAAATGCCAACTTTTAGCGCACGGCTCTCTCCAAGAGCCATTTCCCTGGACAGAATATAGGAATCGTATTC
G

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16480.80 Da        Isoelectric Point: 10.2498

>AT233911 WP_004026357.1 NZ_CP091583:187854-188297 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT233911 NZ_LN854557:c2137367-2137267 [Sodalis glossinidius str. 'morsitans']
TTTCAATCAGATAAAAAAATCGAATACGATTCCTATATTCTGTCCAGGGAAATGGCTCTTGGAGAGAGCCGTGCGCTAAA
AGTTGGCATTTTTGTAGGCTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A663AYG0

Antitoxin

Source ID Structure
AlphaFold DB A0A6P1V3Q9

References