Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 1763773..1764398 Replicon chromosome
Accession NZ_CP091582
Organism Klebsiella pneumoniae strain Nord9_R85

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag L5468_RS08640 Protein ID WP_002882817.1
Coordinates 1763773..1764156 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag L5468_RS08645 Protein ID WP_004150355.1
Coordinates 1764156..1764398 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L5468_RS08625 (L5468_08625) 1761139..1762041 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
L5468_RS08630 (L5468_08630) 1762038..1762673 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
L5468_RS08635 (L5468_08635) 1762670..1763599 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
L5468_RS08640 (L5468_08640) 1763773..1764156 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
L5468_RS08645 (L5468_08645) 1764156..1764398 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
L5468_RS08650 (L5468_08650) 1764603..1765520 + 918 WP_002882812.1 alpha/beta hydrolase -
L5468_RS08655 (L5468_08655) 1765534..1766475 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
L5468_RS08660 (L5468_08660) 1766520..1766957 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
L5468_RS08665 (L5468_08665) 1766954..1767814 - 861 WP_002882807.1 virulence factor BrkB family protein -
L5468_RS08670 (L5468_08670) 1767808..1768407 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T233897 WP_002882817.1 NZ_CP091582:c1764156-1763773 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T233897 NZ_LN850107:c5232022-5231621 [Alloactinosynnema sp. L-07]
GTGATCTATCTGGACAGCTGTGCGATCGTCAAGCTCGTCATACCGGAAGCCGAGTCGCGGGCGCTGAACGTCTGGCTGGC
TGAGCGACCCGATGACCGGTTGGTGACATCGAAGCTCTCTGAGGTCGAAGTGACCCGGGCGGTACGCCGGAGCAGGCCAG
GTGTACTGGGTGGGGTCTCGACGGTGCTGAGGTACCTGGGGCGACTGGAGATCGGCGACGCGGTTCGTGCGACTGCAGCC
GCGTACCTCGATCCCAATCTGCGCACACTTGATGCGATCCATCTGGCCACGGCCCAATCTCTTGAGCTGGCCGGCGGTCC
GCTGTCCGCGTTCGTGACCTACGACAAGCGGCTCGCCGCGGCGGCGGAGGAAGTCGGGCTGCCGGTTGTGGCGCCGAGTT
GA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT233897 WP_004150355.1 NZ_CP091582:c1764398-1764156 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT233897 NZ_LN850107:c5232291-5232019 [Alloactinosynnema sp. L-07]
GTGGAGCAGATACCGATCAGTCAGCTCAATCAAGACACTGCGGGCGCGCTGGCGCGGGTCGCGAACGGCGAGTCGCTGGA
AGTGACCAACCGTGGCAGAGTGGTGGCGCGGCTGGTACCGCCTGCTGATGACGCGTACTCCCGGCTCGTCGCCACCGGGT
TGCTTGCCCCGGCCACGCTCCGCGGCCCGATCCCGATGCCGGAAGGCGAGATCGACGAGACCGGTGCGGCCAGTGCCGCG
CTGATCCAGATGCGCGAGGAAGAGCGCTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References