Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 1181452..1182262 Replicon chromosome
Accession NZ_CP091582
Organism Klebsiella pneumoniae strain Nord9_R85

Toxin (Protein)


Gene name KacT Uniprot ID A0A0H3GMP0
Locus tag L5468_RS05855 Protein ID WP_002887280.1
Coordinates 1181452..1181985 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag L5468_RS05860 Protein ID WP_002887278.1
Coordinates 1181996..1182262 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L5468_RS05850 (L5468_05850) 1180283..1181404 + 1122 WP_002887282.1 cupin domain-containing protein -
L5468_RS05855 (L5468_05855) 1181452..1181985 - 534 WP_002887280.1 type II toxin-antitoxin system toxin KacT Toxin
L5468_RS05860 (L5468_05860) 1181996..1182262 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
L5468_RS05865 (L5468_05865) 1182365..1183798 - 1434 WP_002887275.1 Cu(+)/Ag(+) sensor histidine kinase -
L5468_RS05870 (L5468_05870) 1183788..1184471 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
L5468_RS05875 (L5468_05875) 1184643..1186028 + 1386 WP_002887267.1 efflux transporter outer membrane subunit -
L5468_RS05880 (L5468_05880) 1186046..1186390 + 345 WP_002887266.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19838.70 Da        Isoelectric Point: 5.2614

>T233895 WP_002887280.1 NZ_CP091582:c1181985-1181452 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T233895 NZ_LN849008:1863019-1863270 [Bordetella pertussis]
ATTGGAGGCGCGGCGACAAAAAAGCCGGGACAAAAGCGTCGCGCCGGGGCGGCGGCGCTGGGGCTGGATTTCAGCGCCAT
GGTCGAAATCGTGCTTGCATTGACACCGCGGGATTTCCACAAAAGCATGACTACCCATGCCGACCATCGCATTTGGCAGG
ACGTCTATTGCCCGGTGACAGCGATGGGCGCCGTCTACCTGAAGCTGACGGTCCTCGACGGCGTGCCGATCGTGTCGTTC
AAGGAGTTGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT233895 WP_002887278.1 NZ_CP091582:c1182262-1181996 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT233895 NZ_LN849008:1863273-1863674 [Bordetella pertussis]
ATGAAGTGTCCCGTATGCGGAGCGGCCGCGCTGGCGCGTGATACTCGCGATGTGCCCTATAGCTACAAGGGCGAGACCAT
CACGATTGCGGCGGTGACGGGCGACTGGTGTCCGGCATGCGGCGAGAACGTGCTCGATGGCGCTGAATCGGCGCGGGTGA
GCGAGGCCATGCTGGCTTTCAACCGCCTGGTCAACGCCCGCCGCGTCGACCCCGGGTTCATCGTGCGGGTGCGCAGGAAG
CTGGGGCTGGACCAGCGCGAAGCCGCGGCGCTGTTCGGCGGCGGCACCAATGCGTTCTCGCGTTACGAGAACGGCAAGAC
GCGCCCGCCGCTGGCGCTGGTGAAGCTGCTCAGGCTGCTGGATCGCCATCCGGAGCTGCTGGAGGAAGTCCGTGCGGATT
GA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5ZGN
PDB 5XUN

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References