Detailed information of TA system TA233893

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB-HicA
• Location: 191292..191909
• Replicon: plasmid pUK040_1
• Accession: NZ_CP091578
• Organism: Enterococcus faecium strain UK040

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: L5466_RS14240
• Protein ID: WP_224451579.1
• Coordinates: 191757..191909 (-)
• Length: 51 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A829EWE8
• Locus tag: L5466_RS14235
• Protein ID: WP_002305052.1
• Coordinates: 191292..191723 (-)
• Length: 144 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L5466_RS14220 (L5466_14220)	187107..188534	-	1428	WP_002361237.1	glycoside hydrolase family 32 protein	-
L5466_RS14225 (L5466_14225)	188588..189862	-	1275	WP_002323715.1	MFS transporter	-
L5466_RS14230 (L5466_14230)	190165..191118	+	954	WP_002325884.1	IS30 family transposase	-
L5466_RS14235 (L5466_14235)	191292..191723	-	432	WP_002305052.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
L5466_RS14240 (L5466_14240)	191757..191909	-	153	WP_224451579.1	type II toxin-antitoxin system HicA family toxin	Toxin
L5466_RS14245 (L5466_14245)	192003..192182	-	180	Protein_209	hypothetical protein	-
L5466_RS14250 (L5466_14250)	192198..192398	-	201	WP_002324500.1	hypothetical protein	-
L5466_RS14255 (L5466_14255)	192563..193258	-	696	WP_002305050.1	hypothetical protein	-
L5466_RS14260 (L5466_14260)	193251..193688	-	438	WP_002305049.1	hypothetical protein	-
L5466_RS14265 (L5466_14265)	193704..193955	-	252	WP_002287517.1	hypothetical protein	-
L5466_RS14270 (L5466_14270)	193955..194161	-	207	WP_002324499.1	hypothetical protein	-
L5466_RS14275 (L5466_14275)	194609..194809	-	201	WP_227005125.1	hypothetical protein	-
L5466_RS14280 (L5466_14280)	195001..195145	+	145	Protein_216	putative holin-like toxin	-
L5466_RS14285 (L5466_14285)	195304..195561	-	258	WP_002295625.1	hypothetical protein	-
L5466_RS14290 (L5466_14290)	195583..196068	-	486	Protein_218	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..231428	231428
	-	flank	IS/Tn	-	-	190165..191118	953

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (2-49)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (10-104)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5815.65 Da
• Isoelectric Point: 10.3560

>T233893 WP_224451579.1 NZ_CP091578:c191909-191757 [Enterococcus faecium]
IEKNGWVERRQEGFHHHLYKDGVRITVPVHANQDLGRGLERKILKDAGLK

Nucleotide

• Length: 153 bp

>T233893 NZ_LN847353:c1532942-1532763 [Streptococcus pneumoniae]
ATGCCTATGACACAAAAAGAGATGGTTAAACTCCTTACGGCCCATGGTTGGATAAAGACTAGAGGCGGTAAAGGCTCCCA
TATTAAAATGGAAAAGCAAGGGGAAAGACCTATCACAATCCTTCATGGTGAGCTGAATAAGTACACTGAAAGAGGGATAA
GAAAGCAAGCTGGGTTGTAA

Antitoxin

Protein

• Length: 144 a.a.
• Molecular weight: 15811.92 Da
• Isoelectric Point: 4.1638

>AT233893 WP_002305052.1 NZ_CP091578:c191723-191292 [Enterococcus faecium]
MLSEPLAKTYPAIFSPEEGGGYFIEFPDVQGAYTGINEDDISYGIAMAQEVLGMVLADYIEHEDLLPEPTPINKISVEDD
SFTTLIRVDVAKYLKDTELVKKTLTIPQWADKLGKRAGINFSVLLTESIADKADNILRSGRNN

Nucleotide

• Length: 432 bp

>AT233893 NZ_LN847353:c1532726-1532274 [Streptococcus pneumoniae]
ATGTTAGTTACGTATCCAGCTTTATTTTATTATGACGACACAGACGGAACAGAAGCGACTTATTTTGTCCATTTCCCAGA
TTTTGAATACTCAGCTACACAAGGAGAGGGGATTTCTGAGGCTTTGGCTATGGGGTCGGAGTGGCTAGGGATAACTGTTG
CAGATTTGATAGAGAGTGATGGAGAGTTACCTCAGCCATCAGATATCAATAGCTTGTCTTTAATTGATAATGATCCTTTT
AAAGATGATGAAGATTTCGTGTCAACCTATGACCTTGATAAATCTTTTATTTCTATGGTATCTGTTGATGTATCAGAATA
CCTAGGAAGTCAGGAACCCATTAAAAAGACTTTGACCATACCAAAATGGGCAGATAAGTTGGGACGAGAAATGGGACTTA
ACTTTTCTCAGACTTTGACAGACGCTATTGCAGATAAGAAAGTTCAAGCCTAA

Structures

Antitoxin

• AlphaFold DB: A0A829EWE8