Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 1592137..1592708 Replicon chromosome
Accession NZ_CP091577
Organism Enterococcus faecium strain UK040

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag L5466_RS07830 Protein ID WP_002286801.1
Coordinates 1592137..1592478 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag L5466_RS07835 Protein ID WP_002323011.1
Coordinates 1592478..1592708 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L5466_RS07805 (L5466_07805) 1587347..1588681 - 1335 WP_002286788.1 ABC transporter substrate-binding protein -
L5466_RS07810 (L5466_07810) 1588889..1589719 + 831 WP_002286789.1 manganese catalase family protein -
L5466_RS07815 (L5466_07815) 1589963..1590212 - 250 Protein_1528 transposase -
L5466_RS07820 (L5466_07820) 1590403..1590597 - 195 WP_002297028.1 hypothetical protein -
L5466_RS07825 (L5466_07825) 1591102..1591287 - 186 WP_002304207.1 hypothetical protein -
L5466_RS07830 (L5466_07830) 1592137..1592478 - 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
L5466_RS07835 (L5466_07835) 1592478..1592708 - 231 WP_002323011.1 hypothetical protein Antitoxin
L5466_RS07840 (L5466_07840) 1592995..1593333 + 339 WP_002286804.1 hypothetical protein -
L5466_RS07845 (L5466_07845) 1593538..1594100 - 563 Protein_1534 SOS response-associated peptidase family protein -
L5466_RS07850 (L5466_07850) 1594832..1595413 - 582 WP_002286813.1 TetR/AcrR family transcriptional regulator -
L5466_RS07855 (L5466_07855) 1595596..1596222 - 627 WP_002286816.1 cysteine hydrolase -
L5466_RS07860 (L5466_07860) 1596244..1597575 - 1332 WP_002286818.1 FAD-containing oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T233890 WP_002286801.1 NZ_CP091577:c1592478-1592137 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T233890 NZ_LN847264:c3599817-3599533 [Pseudomonas sp. CCOS 191]
ATGACTTATAAGCTCGAGTTTCTGCCTTCGGCGCTGAAAGAATGGAAGAAGCTGGGGCACACCGTGCGCGAGCAAGCCAG
GAGAAAACTCAAGGAGCGTCTGCTCTCGCCCAGGGTTGGGGCGGATGCAATGCGTGATTTGCCTGACCATTTCAAGATCA
AGCTCAAGGCCAGCGGCTATCGGGTGGTCTACCGCGTCGAGGATGATCGGGTAGTTGTCGTGGTCGTGGCGATAGGCAAG
CGCGAACGTGGGCAAGCCTACCGCTCGGCCGCTGATCGCCTATAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT233890 WP_002323011.1 NZ_CP091577:c1592708-1592478 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT233890 NZ_LN847264:c3600052-3599807 [Pseudomonas sp. CCOS 191]
ATGCAGAACATCTTTGCCGACGTGACCGTCAGCGTCTCGGAACTAAAGAAGAATCCTACGGCGGTGATGGCCGATGCGCA
GGGGAAGCCGGTGGCCGTTCTCAACCATAATCGCGTCATGGGTTACATGGTGCCCGCTGAACTCTACGAGGCGATGATGG
AGCGACTGGAAGACATGGAACTTGCGGCATTGGTAAAGGCGCGTGCGGGAGAGAAAGGCATCCCGGTCGAACTGGATGAC
TTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References