Detailed information of TA system TA233874

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 4145437..4146013
• Replicon: chromosome
• Accession: NZ_CP091571
• Organism: Salmonella enterica strain

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: M7RG88
• Locus tag: INN74_RS20315
• Protein ID: WP_001131963.1
• Coordinates: 4145726..4146013 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: B5R9R5
• Locus tag: INN74_RS20310
• Protein ID: WP_000063142.1
• Coordinates: 4145437..4145739 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
INN74_RS20295 (4141947)	4141947..4144097	+	2151	WP_000379927.1	pyruvate/proton symporter BtsT	-
INN74_RS20300 (4144192)	4144192..4144395	+	204	WP_000460616.1	YbdD/YjiX family protein	-
INN74_RS20305 (4144406)	4144406..4145362	+	957	WP_000187839.1	GTPase	-
INN74_RS20310 (4145437)	4145437..4145739	-	303	WP_000063142.1	BrnA antitoxin family protein	Antitoxin
INN74_RS20315 (4145726)	4145726..4146013	-	288	WP_001131963.1	BrnT family toxin	Toxin
INN74_RS20320 (4146282)	4146282..4147196	-	915	WP_000290512.1	restriction endonuclease	-
INN74_RS20325 (4147394)	4147394..4150903	+	3510	WP_001043484.1	type I restriction-modification system endonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11294.80 Da
• Isoelectric Point: 9.5894

>T233874 WP_001131963.1 NZ_CP091571:c4146013-4145726 [Salmonella enterica]
MPMEFEWDANKAKSNLRKHGVRFEDAVLVFDDPRHLSRQERYENGEYRWQTLGLVHGIVVILVAHSVRFESGFEVIRIIS
ARKADRKERNRYEHG

Nucleotide

• Length: 288 bp

>T233874 NZ_LN832404:c2907395-2907060 [Escherichia coli K-12]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11373.96 Da
• Isoelectric Point: 10.1293

>AT233874 WP_000063142.1 NZ_CP091571:c4145739-4145437 [Salmonella enterica]
MSMVKHKRGNASALSAQHEAELKALAKKSDDEIDYSDIPASEDGQWSEAVRGKFFRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLREQNKK

Nucleotide

• Length: 303 bp

>AT233874 NZ_LN832404:c2907643-2907395 [Escherichia coli K-12]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• PDB: 7VD7

Antitoxin

• AlphaFold DB: A0A4D6PB01