Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/RelE-RHH
Location 84490..85037 Replicon plasmid pECOS88
Accession NC_011747
Organism Escherichia coli S88

Toxin (Protein)


Gene name parE Uniprot ID E9LLH3
Locus tag ECS88_RS00550 Protein ID WP_000969996.1
Coordinates 84756..85037 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID E1QMW3
Locus tag ECS88_RS00545 Protein ID WP_000079941.1
Coordinates 84490..84759 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECS88_RS00495 79764..80510 + 747 WP_000205759.1 conjugal transfer pilus acetylase TraX -
ECS88_RS00500 80565..81125 + 561 WP_000139359.1 fertility inhibition protein FinO -
ECS88_RS00505 81263..81475 + 213 WP_001311693.1 hypothetical protein -
ECS88_RS00510 81718..82119 + 402 WP_001233870.1 thermonuclease family protein -
ECS88_RS00515 82164..82424 + 261 WP_000083818.1 replication regulatory protein RepA -
ECS88_RS00525 82650..82724 + 75 WP_001365705.1 RepA leader peptide Tap -
ECS88_RS00530 82717..83574 + 858 WP_000130940.1 incFII family plasmid replication initiator RepA -
ECS88_RS00545 84490..84759 + 270 WP_000079941.1 hypothetical protein Antitoxin
ECS88_RS00550 84756..85037 + 282 WP_000969996.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
ECS88_RS00555 85083..85931 + 849 WP_001057989.1 3'-5' exonuclease -
ECS88_RS00560 86117..87997 + 1881 WP_001283335.1 colicin -
ECS88_RS00565 88019..88354 - 336 WP_001080729.1 colicin 1A immunity protein -
ECS88_RS00575 88483..88740 + 258 WP_000142431.1 hypothetical protein -
ECS88_RS00580 88760..89350 - 591 WP_001350923.1 hypothetical protein -
ECS88_RS00585 89347..89607 - 261 WP_000343091.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD iutA / iucD / iucC / iucB / iucA / iroN / iroE / iroD / iroC / iroB 1..133853 133853


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-92)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10893.57 Da        Isoelectric Point: 8.4615

>T23386 WP_000969996.1 NC_011747:84756-85037 [Escherichia coli S88]
MMEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Download         Length: 282 bp

>T23386 NC_011747:84756-85037 [Escherichia coli S88]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
GGCCGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTG
TATATCCTGCGTGTATTGCATACTGCACAGAAGTGGCCATAG

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10252.58 Da        Isoelectric Point: 7.2057

>AT23386 WP_000079941.1 NC_011747:84490-84759 [Escherichia coli S88]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRD
EMSRKVAGK

Download         Length: 270 bp

>AT23386 NC_011747:84490-84759 [Escherichia coli S88]
ATGGCACAGGTTAATATGAGTTTAAGAATTGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTGGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCAGGACGTCAGCAACTCGAGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCCGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAAGTCGCCGGTAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB E9LLH3

Antitoxin

Source ID Structure
AlphaFold DB A0A629TXC5

References