Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 51383..52026 Replicon plasmid pEFER
Accession NC_011743
Organism Escherichia fergusonii ATCC 35469

Toxin (Protein)


Gene name vagD Uniprot ID Q84A06
Locus tag EFER_RS00220 Protein ID WP_000754566.1
Coordinates 51383..51799 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag EFER_RS00225 Protein ID WP_001261276.1
Coordinates 51796..52026 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EFER_RS00195 47864..48667 + 804 WP_001082319.1 aminoglycoside O-phosphotransferase APH(3'')-Ib -
EFER_RS00200 48667..49503 + 837 WP_000480968.1 aminoglycoside O-phosphotransferase APH(6)-Id -
EFER_RS00205 49610..50041 - 432 Protein_43 IS110 family transposase -
EFER_RS23970 50323..51179 - 857 Protein_44 IS3 family transposase -
EFER_RS00220 51383..51799 - 417 WP_000754566.1 type II toxin-antitoxin system VapC family toxin Toxin
EFER_RS00225 51796..52026 - 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
EFER_RS00235 52600..52950 + 351 WP_000493378.1 hypothetical protein -
EFER_RS00240 53001..53744 + 744 WP_000129823.1 hypothetical protein -
EFER_RS00245 53741..54517 + 777 WP_000015958.1 site-specific integrase -
EFER_RS00250 54575..54832 - 258 WP_000764641.1 hypothetical protein -
EFER_RS24400 54961..55065 - 105 WP_032409716.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaTEM-1B / tet(B) / aph(3'')-Ib / aph(6)-Id - 1..55150 55150


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15006.52 Da        Isoelectric Point: 9.2957

>T23383 WP_000754566.1 NC_011743:c51799-51383 [Escherichia fergusonii ATCC 35469]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Download         Length: 417 bp

>T23383 NC_011743:c51799-51383 [Escherichia fergusonii ATCC 35469]
ATGAAGAAAACCTGGATGCTCGACACCAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATTGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCGTCCTGCCCTGGGACCGCGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTAGCCGGGACGCCGATCGGCCCGAATGACACGGCCATTGC
CGGGCACGCCATCGCGGCGGGCGCCATCCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTCCCGGGCCTGGTGCTGG
AAGACTGGGTGAAATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT23383 WP_001261276.1 NC_011743:c52026-51796 [Escherichia fergusonii ATCC 35469]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT23383 NC_011743:c52026-51796 [Escherichia fergusonii ATCC 35469]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGATGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A387K1G3

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References