Detailed information of TA system TA233829

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 26277..26869
• Replicon: plasmid p137-mcr
• Accession: NZ_CP091567
• Organism: Salmonella enterica strain 137

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: V0AGQ2
• Locus tag: INN76_RS24540
• Protein ID: WP_000520549.1
• Coordinates: 26277..26525 (+)
• Length: 83 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: V0AWG0
• Locus tag: INN76_RS24545
• Protein ID: WP_000681613.1
• Coordinates: 26522..26869 (+)
• Length: 116 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
INN76_RS24510 (INN76_24500)	23154..23669	+	516	WP_001025395.1	J domain-containing protein	-
INN76_RS24515 (INN76_24505)	23774..24316	+	543	WP_001083906.1	hypothetical protein	-
INN76_RS24520 (INN76_24510)	24313..24564	+	252	WP_001230707.1	hypothetical protein	-
INN76_RS24525 (INN76_24515)	24551..24778	+	228	WP_000801440.1	hypothetical protein	-
INN76_RS24530 (INN76_24520)	25232..25531	+	300	WP_000650309.1	hypothetical protein	-
INN76_RS24535 (INN76_24525)	25622..25972	+	351	WP_000854259.1	hypothetical protein	-
INN76_RS24540 (INN76_24530)	26277..26525	+	249	WP_000520549.1	type II toxin-antitoxin system HicA family toxin	Toxin
INN76_RS24545 (INN76_24535)	26522..26869	+	348	WP_000681613.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
INN76_RS24550 (INN76_24540)	27230..27733	+	504	WP_000781813.1	relaxosome-coupling protein TaxA	-
INN76_RS24555 (INN76_24545)	27737..28966	+	1230	WP_001329504.1	DNA relaxase/nickase TaxC	-
INN76_RS24560 (INN76_24550)	29043..29336	+	294	WP_060598542.1	sigma factor-like helix-turn-helix DNA-binding protein	-
INN76_RS24565 (INN76_24555)	29333..29548	+	216	WP_000760375.1	EexN family lipoprotein	-
INN76_RS24570 (INN76_24560)	29904..30524	+	621	WP_249004921.1	lytic transglycosylase domain-containing protein	-
INN76_RS24575 (INN76_24565)	30538..30864	+	327	WP_000008647.1	TrbC/VirB2 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	mcr-1.1	-	1..33309	33309

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (20-81)

Antitoxin

• Uncharacterised protein family HicB (54-104)

Sequences

Toxin

Protein

• Length: 83 a.a.
• Molecular weight: 9422.08 Da
• Isoelectric Point: 10.4800

>T233829 WP_000520549.1 NZ_CP091567:26277-26525 [Salmonella enterica]
MGKTDKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
LL

Nucleotide

• Length: 249 bp

>T233829 NZ_LN831035:377429-377650 [Haemophilus influenzae]
GTGCAAAATCTCCTTGAAAATCAAGCTCTTTTTGCTATTTTAGACGGTGAAATTTGGGAACTTCGCCCAATTCGGGATCG
CATCTTGTTTGCAAGATTAATGGACGGTCGCTTTGTCTTACTACATCAATTTATGAAGAAAACACAAAAAACGCCAAAAC
GAGAAATCCAAACTGCCCAACAACGCTTAAGTGAATTAAAAGAGAGATTGAAAAATGAATAA

Antitoxin

Protein

• Length: 116 a.a.
• Molecular weight: 13113.84 Da
• Isoelectric Point: 4.6723

>AT233829 WP_000681613.1 NZ_CP091567:26522-26869 [Salmonella enterica]
MKHLKYKGYLGTVEPDFENNVLYGKLAFIRDLVTYEASTLAELEQEFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSNHRAGA

Nucleotide

• Length: 348 bp

>AT233829 NZ_LN831035:377643-377939 [Haemophilus influenzae]
ATGAATAAAATCAGCCCATTAGGTTCAAATTGGAATGAGTTTGAACAGCAAATTTTCAATGAAGAAGAAATCCGAGAAAG
TAATTTGCGTGTAGCCTTAATTAAAGAATTGATTACTTCTCGTCAGCAACTTGGGATTTCCCAAAAACAACTTGAAACCT
TAAGCGGTGTGAAGCAACCTATGATTGCACGCATTGAAAAAGGACAAACGAATCCTCAACTTGAAACGTTGTTAAAATTG
CTCGCTCCTTTAGGGAAAACCCTGTCGATTGTGCCTCTAAGGGTAAAAAATGCCTAA

Structures

Toxin

• AlphaFold DB: A0A829CIX3

Antitoxin

• AlphaFold DB: A0A829CLC1