Detailed information of TA system TA233828

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsxAB/MqsA(antitoxin)
• Location: 24313..24778
• Replicon: plasmid p137-mcr
• Accession: NZ_CP091567
• Organism: Salmonella enterica strain 137

Toxin (Protein)

• Gene name: TsxA
• Uniprot ID: U9ZUY2
• Locus tag: INN76_RS24520
• Protein ID: WP_001230707.1
• Coordinates: 24313..24564 (+)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: TsxB
• Uniprot ID: M7CCF8
• Locus tag: INN76_RS24525
• Protein ID: WP_000801440.1
• Coordinates: 24551..24778 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
INN76_RS24495 (INN76_24485)	19794..20321	-	528	WP_000577043.1	DUF2726 domain-containing protein	-
INN76_RS24500 (INN76_24490)	20456..20860	-	405	WP_012421203.1	hypothetical protein	-
INN76_RS24505 (INN76_24495)	20873..21739	-	867	WP_001353743.1	replication initiation protein	-
INN76_RS24510 (INN76_24500)	23154..23669	+	516	WP_001025395.1	J domain-containing protein	-
INN76_RS24515 (INN76_24505)	23774..24316	+	543	WP_001083906.1	hypothetical protein	-
INN76_RS24520 (INN76_24510)	24313..24564	+	252	WP_001230707.1	hypothetical protein	Toxin
INN76_RS24525 (INN76_24515)	24551..24778	+	228	WP_000801440.1	hypothetical protein	Antitoxin
INN76_RS24530 (INN76_24520)	25232..25531	+	300	WP_000650309.1	hypothetical protein	-
INN76_RS24535 (INN76_24525)	25622..25972	+	351	WP_000854259.1	hypothetical protein	-
INN76_RS24540 (INN76_24530)	26277..26525	+	249	WP_000520549.1	type II toxin-antitoxin system HicA family toxin	-
INN76_RS24545 (INN76_24535)	26522..26869	+	348	WP_000681613.1	type II toxin-antitoxin system HicB family antitoxin	-
INN76_RS24550 (INN76_24540)	27230..27733	+	504	WP_000781813.1	relaxosome-coupling protein TaxA	-
INN76_RS24555 (INN76_24545)	27737..28966	+	1230	WP_001329504.1	DNA relaxase/nickase TaxC	-
INN76_RS24560 (INN76_24550)	29043..29336	+	294	WP_060598542.1	sigma factor-like helix-turn-helix DNA-binding protein	-
INN76_RS24565 (INN76_24555)	29333..29548	+	216	WP_000760375.1	EexN family lipoprotein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	mcr-1.1	-	1..33309	33309

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (8-107)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (5-93)

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9751.13 Da
• Isoelectric Point: 8.9187

>T233828 WP_001230707.1 NZ_CP091567:24313-24564 [Salmonella enterica]
MRKNKGDVTYFLEKEGDNYRLTKRIKARTNVKIGNKTTKITLYDAVLNENELQHIDFTCAGLREDDETPVKNLIKEFMLN
ETR

Nucleotide

• Length: 252 bp

>T233828 NZ_LN831035:c322791-322456 [Haemophilus influenzae]
ATGTCAGAAGAGAAGCCATTAAAAGTAAGCTATTCAAAACAATTTGTAAGAGATTTAACTGATTTAGCTAAACGCTCCCC
CAATGTGTTAATTGGCTCAAAATACATTACTGCAATATACTGTTTATTAAATCGTTTACCTTTACCCGAAAACTACCAAG
ATCACGCATTAGTAGGAGAGTGGAAAGGTTATCGAGATTGCTATATTCAAGGCAATTTGGTACTGATTTACCAATACGTT
ATACGCGATGAATTTGATGAATTTGATGAATTTGATGAATTTGATGAATTGAAATTTTCTCGTTTAAATACACACTCACA
AACCGCTTTAAAATAG

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8659.98 Da
• Isoelectric Point: 8.5105

>AT233828 WP_000801440.1 NZ_CP091567:24551-24778 [Salmonella enterica]
MKPDNTPENVKKLRLKAGLTQKECSHIYGVGLRTWQKKEEVNTQNSQSLSLVEFEFLLLLAGEHPEYVLCKRESK

Nucleotide

• Length: 228 bp

>AT233828 NZ_LN831035:c323087-322791 [Haemophilus influenzae]
ATGGCACTTACGAATTCCTCTATCAGCTTTAGAACAGTTGAGCAAACAAAATCAGAAGCTTATCAAGTTATTGAACAATA
TGGACTTACCCCATCACAAGTATTCAATATGTTTTTGGCTCAAATTGCCAAGACTCGCTCTATCCCTATTGATTTGAATT
ATCTTCGCCCAAATAAAGAAACCCTAGCAGCTATTGATGAATTAGATAGTGGTAACGCAGAGAGCTTTTTTATTGAAGCT
AGTGAAAATTATTCTGCCGAAGAATTTACAAAACGCATTCTTAATGGTGGTCAATAA

Structures

Toxin

• AlphaFold DB: U9ZUY2

Antitoxin

• AlphaFold DB: A0A5Y6U8W9