233722

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 90172..91795 Replicon plasmid p632-mcr
Accession NZ_CP091557
Organism Salmonella enterica strain 632

Toxin (Protein)


Gene name hipA Uniprot ID H9TH67
Locus tag INN80_RS24610 Protein ID WP_000088046.1
Coordinates 90470..91795 (+) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A5Z6V2D1
Locus tag INN80_RS24605 Protein ID WP_024136327.1
Coordinates 90172..90465 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
INN80_RS24580 (INN80_24575) 86440..87414 - 975 WP_012478345.1 IS30-like element ISApl1 family transposase -
INN80_RS24585 (INN80_24580) 87848..88129 - 282 WP_000077926.1 hypothetical protein -
INN80_RS24590 (INN80_24585) 88179..88370 - 192 WP_000398480.1 hypothetical protein -
INN80_RS24595 (INN80_24590) 88462..88803 - 342 WP_001151575.1 hypothetical protein -
INN80_RS24600 (INN80_24595) 89176..89568 + 393 WP_000341066.1 hypothetical protein -
INN80_RS24605 (INN80_24600) 90172..90465 + 294 WP_024136327.1 helix-turn-helix transcriptional regulator Antitoxin
INN80_RS24610 (INN80_24605) 90470..91795 + 1326 WP_000088046.1 type II toxin-antitoxin system HipA family toxin Toxin
INN80_RS24615 (INN80_24610) 91856..92062 + 207 WP_000134171.1 hypothetical protein -
INN80_RS24620 (INN80_24615) 92164..92574 + 411 WP_000985911.1 hypothetical protein -
INN80_RS24625 (INN80_24620) 92587..93402 + 816 WP_001572439.1 HNH endonuclease signature motif containing protein -
INN80_RS24630 (INN80_24625) 93656..94081 + 426 WP_001043843.1 hypothetical protein -
INN80_RS24635 (INN80_24630) 94535..94770 + 236 Protein_91 type I restriction-modification system subunit M N-terminal domain-containing protein -
INN80_RS24640 (INN80_24635) 94825..95124 + 300 WP_001572440.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid mcr-1.1 / fosA3 / blaCTX-M-14 / aac(3)-IVa / aph(4)-Ia / sul2 / floR / aadA2 / cmlA1 / ant(3'')-Ia / sul3 / sul1 / oqxA / oqxB - 1..221549 221549
- flank IS/Tn mcr-1.1 - 84619..87363 2744


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(9-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50249.20 Da        Isoelectric Point: 6.1927

>T233722 WP_000088046.1 NZ_CP091557:90470-91795 [Salmonella enterica]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

>T233722 NZ_LN614756:c2622721-2622569 [Clostridioides difficile]
ATGGATAATTTTTTATTTAATGTGTTAGCTAGTGTAACAGCTAGTTACATAGTTTATTTGATTAGCAAGTTAATCAAAAA
AGTAAAAAGCCACTCTACCGCAAAGAGTGACTTAAAGATTGATATAAATTTTAAATATCATCGTAAAAAATAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10946.35 Da        Isoelectric Point: 10.0925

>AT233722 WP_024136327.1 NZ_CP091557:90172-90465 [Salmonella enterica]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Download         Length: 294 bp

>AT233722 NZ_LN614756:2622519-2622648 [Clostridioides difficile]
AAGAAGAACTACAATCTATTTGCGGTAGAGTGAAGTTCTATAACTGAAAGTTATTTTTTACGATGATATTTAAAATTTAT
ATCAATCTTTAAGTCACTCTTTGCGGTAGAGTGGCTTTTTACTTTTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3T0VBU1

Antitoxin

Source ID Structure
AlphaFold DB A0A5Z6V2D1

References