Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 88836..90459 Replicon plasmid p751-mcr
Accession NZ_CP091555
Organism Salmonella enterica strain 751

Toxin (Protein)


Gene name hipA Uniprot ID H9TH67
Locus tag INN81_RS24790 Protein ID WP_000088046.1
Coordinates 89134..90459 (+) Length 442 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A5Z6V2D1
Locus tag INN81_RS24785 Protein ID WP_024136327.1
Coordinates 88836..89129 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
INN81_RS24760 (INN81_24755) 85104..86078 - 975 WP_012478345.1 IS30-like element ISApl1 family transposase -
INN81_RS24765 (INN81_24760) 86512..86793 - 282 WP_000077926.1 hypothetical protein -
INN81_RS24770 (INN81_24765) 86843..87034 - 192 WP_000398480.1 hypothetical protein -
INN81_RS24775 (INN81_24770) 87126..87467 - 342 WP_001151575.1 hypothetical protein -
INN81_RS24780 (INN81_24775) 87840..88232 + 393 WP_000341066.1 hypothetical protein -
INN81_RS24785 (INN81_24780) 88836..89129 + 294 WP_024136327.1 helix-turn-helix transcriptional regulator Antitoxin
INN81_RS24790 (INN81_24785) 89134..90459 + 1326 WP_000088046.1 type II toxin-antitoxin system HipA family toxin Toxin
INN81_RS24795 (INN81_24790) 90520..90726 + 207 WP_000134171.1 hypothetical protein -
INN81_RS24800 (INN81_24795) 90828..91238 + 411 WP_000985911.1 hypothetical protein -
INN81_RS24805 (INN81_24800) 91251..92066 + 816 WP_001572439.1 HNH endonuclease signature motif containing protein -
INN81_RS24810 (INN81_24805) 92320..92745 + 426 WP_001043843.1 hypothetical protein -
INN81_RS24815 (INN81_24810) 93199..93434 + 236 Protein_90 type I restriction-modification system subunit M N-terminal domain-containing protein -
INN81_RS24820 (INN81_24815) 93489..93788 + 300 WP_001572440.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid mcr-1.1 / fosA3 / blaCTX-M-14 / aac(3)-IVa / aph(4)-Ia / sul2 / floR / sul1 / dfrA12 / sul3 / ant(3'')-Ia / cmlA1 / aadA2 / oqxA / oqxB - 1..249973 249973
- flank IS/Tn mcr-1.1 - 83283..86027 2744


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(9-108)

Antitoxin

Cro/C1-type helix-turn-helix domain

(21-69)


Sequences

Toxin        


Download         Length: 442 a.a.        Molecular weight: 50249.20 Da        Isoelectric Point: 6.1927

>T233697 WP_000088046.1 NZ_CP091555:89134-90459 [Salmonella enterica]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Download         Length: 1326 bp

>T233697 NZ_LM995446:c1594530-1594243 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10946.35 Da        Isoelectric Point: 10.0925

>AT233697 WP_024136327.1 NZ_CP091555:88836-89129 [Salmonella enterica]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Download         Length: 294 bp

>AT233697 NZ_LM995446:c1594769-1594530 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3T0VBU1

Antitoxin

Source ID Structure
AlphaFold DB A0A5Z6V2D1

References